Microscopic and molecular identification of hemotropic mycoplasmas in South American coatis (Nasua nasua) |
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| Institution: | 1. Department of Cell and Molecular Biology, Universidade Federal do Parana, Av. Cel. Francisco H. dos Santos, s/n., Curitiba, PR 81531-980, Brazil;2. Bela Vista Biological Sanctuary, Itaipu Binacional, R. Teresina, 62, Foz do Iguacu, PR 85866-900, Brazil;3. Department of Veterinary Medicine, Universidade Federal do Parana, R. dos Funcionarios, 1540, Curitiba, PR 80035-050, Brazil;4. IDEXX Laboratories Inc., 2825 KOVR Drive, West Sacramento, CA 95605, USA;5. Department of Medicine & Epidemiology, School of Veterinary Medicine, University of California, 2108 Tupper Hall, Davis, CA 95616, USA;1. Department of Veterinary Clinical Pathology, School of Veterinary Medicine, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil;2. Department of Comparative Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, IN, USA;3. School of Agronomy and Veterinary Medicine, Universidade de Passo Fundo, Passo Fundo, Rio Grande do Sul, Brazil;4. Department of Preventive Veterinary Medicine, School of Veterinary Medicine, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil;5. Department of Veterinary Clinical Pathology, Universidade Federal do Paraná, Curitiba, Paraná, Brazil;1. Departament of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina, PR, 86057-970, Brazil;2. Laboratory of Animal Health, Instituto Federal do Maranhão, São Luís, MA, 65095-460, Brazil;3. Departament of Veterinary Medicine, Universidade Federal do Paraná, Curitiba, PR, 80035-050, Brazil;1. Pós-graduação em Microbiologia Agropecuária, Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil;2. Laboratório de Imunoparasitologia, Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil;3. Laboratório de Biologia de Tripanosomatídeos, Fundação Oswaldo Cruz/FioCruz, Rio de Janeiro, RJ, Brazil;4. Direcção de Ciências Animais, Maputo, Moçambique;1. Institute of Veterinary Clinical Sciences, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia, Chile;2. Institute of Pharmacology and Morphophysiology, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia, Chile;3. Departament of Pathology and Veterinary Clinical, Faculty of Veterinary Sciences, Universidade Federal Fluminense, Niterói, Brazil;4. Institute of Veterinary Preventive Medicine and Applied Research Program on Wildlife, Faculty of Veterinary Sciences, Universidad Austral de Chile, Valdivia, Chile;1. Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran;2. Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran;3. School of Veterinary Medicine, Shiraz University, Shiraz, Iran;1. Veterinary School, Universidade Federal de Minas Gerais (UFMG), Antônio Carlos Avenue, 6627, Belo Horizonte 31.270-901, MG, Brazil;2. Faculty of Medicine, University of Maribor, Slovenia;3. National Laboratory for Health, Environment and Food, Maribor, Slovenia;4. Center of Excellence for Integrated Approaches in Chemistry and Biology of Proteins, Ljubljana, Slovenia |
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| Abstract: | Hemoplasmas were detected in two apparently healthy captive South American coatis (Nasua nasua) from southern Brazil during an investigation for vector-borne pathogens. Blood was subjected to packed cell volume (PCV) determination, a commercial real-time PCR panel for the detection of Anaplasma spp., Babesia spp., Bartonella spp., Hepatozoon spp., Leishmania spp., Mycoplasma haemofelis, ‘Candidatus Mycoplasma turicensis’, ‘Candidatus Mycoplasma haemominutum’, Neorickettsia risticii, Rickettsia rickettsii and Leptospira spp., and a pan-hemoplasma conventional PCR assay. PCV was normal, but both coatis tested positive for hemoplasmas and negative for all the remaining pathogens tested. Using different techniques for microscopy (light, confocal or SEM), structures compatible with hemoplasmas were identified. Sequencing of the 16S rRNA gene identified an organism resembling Mycoplasma haemofelis and another hemotropic Mycoplasma sp., with a sequence identity of 96.8% to a Mycoplasma sp. previously detected in capybaras. |
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| Keywords: | Hemoplasma Vector-borne disease Wild mammal |
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