Neotropical primary bat cell lines show restricted dengue virus replication |
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| Institution: | 1. Department of Morphology, Faculty of Medicine, Federal University of Ceara, Fortaleza, CE, Brazil;2. Laboratory of Bacteriology, Department of Pathology, Faculty of Medicine, Federal University of Ceara, Fortaleza, CE, Brazil;3. Haroldo Juaçaba Hospital, Cancer Institute of Ceara, Fortaleza, Brazil;4. Laboratory of Pharmacology of Inflammation and Cancer (LAFICA), Department of Physiology and Pharmacology, Federal University of Ceara, Fortaleza, Brazil;5. Laboratorio de Investigación en Bacteriología Anaerobia, Facultad de Microbiología and Centro de Investigación en Enfermedades Tropicales, Universidad de Costa Rica, San José, Costa Rica;1. Centro de Investigación en Enfermedades Tropicales (CIET), Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica;2. Centro de Investigación en Estructuras Microscópicas (CIEMIC), Universidad de Costa Rica, San José, Costa Rica;1. Department of Molecular Medicine, ‘Sapienza’ University of Rome, Rome, Italy;2. Infectious Diseases Unit 3rd Department of Paediatrics Faculty of Medicine, Aristotle University School of Health Sciences, Thessaloniki, Greece;1. Departamento de Parasitología, Centro de Investigación en Enfermedades Tropicales, Facultad de Microbiología, Universidad de Costa Rica, P.O. Box 11501-2060, San José, Costa Rica;2. Departamento de Parasitología, Escuela de Medicina Veterinaria, Universidad Nacional, P.O. Box 86-3000, Heredia, Costa Rica;3. Koret School of Veterinary Medicine, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel |
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| Abstract: | Dengue is the most widespread arboviral disease affecting humans. Bats are recognized carriers of emerging viral zoonoses and have been proposed as dengue reservoirs, since RNA/NS1 and/or antiviral antibodies have been detected. Yet, experimental inoculation of Artibeus bats failed to show virus replication. This conflicting results prevent drawing further conclusions of whether bats sustain dengue infection. To test bat cellular permissivity to dengue infection, we established primary bat embryonic cells from diverse organs and tissues of Artibeus jamaicensis, Molossus sinaloae, and Desmodus rotundus. We observed a limited serotype-, organ-, and bat species- specific dengue susceptibility. Only some Molossus-derived primary cells sustained poorly initial Dengue serotype-1 replication, though it was latter absent. To elucidate if Molossus bats may play a role in dengue replication, ecological or in vivo experiments must be performed. Taken together our results show that Dengue did not replicate efficiently in cell lines derived from Neotropical bat species. |
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| Keywords: | Dengue Viral infection in vitro studies Bats |
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