| 新铁炮百合离体培养的生理生化变化初探 |
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| 引用本文： | 陈丽静,孙春玉,马慧,陶承光,阮燕晔,李天来,王玉坤.新铁炮百合离体培养的生理生化变化初探[J].北方园艺,2011(1):149-152. |
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| 作者姓名： | 陈丽静 孙春玉 马慧 陶承光 阮燕晔 李天来 王玉坤 |
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| 作者单位： | 沈阳农业大学辽宁省生物技术重点实验室设施园艺省部共建教育部重点实验室;辽宁省农业科学院;中国农业科学院蔬菜花卉研究所; |
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| 基金项目： | 国家高技术研究发展计划(863计划)资助项目(2006AA100109); 中国博士后科学基金资助项目(20100471471); 辽宁省教育厅资助项目(L2010495) |
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| 摘 要： | 以离体培养不同时间的新铁炮百合新品种"沈香一号"鳞茎为材料,在MS+6-BA0.5 mg/L+NAA 0.1 mg/L和MS无激素培养基条件下,测定并分析激素对新铁炮百合生理生化特性的影响。结果表明:激素处理的新铁炮再生苗中可溶性蛋白和非可溶性蛋白含量的变化趋势较大,适宜的激素处理能促进可溶性糖和淀粉含量的相互转变,明显增加新铁炮百合组织中过氧化物歧化酶(SOD)含量和降低丙二醛(MDA)含量;在不定芽形成过程中,激素处理可以增强百合分化和脱分化过程中的蛋白和糖代谢,还会提高组织抗逆性。
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| 关 键 词： | 新铁炮百合 鳞茎 激素 生理生化特性 |
Physiological and Biochemical Changes During Regeneration of the Lilium longiflorum Thunb |
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| CHEN Li-jing,SUN Chun-yu,MA Hui,TAO Cheng-guang,RUAN Yan-ye,LI Tian-lai,WANG Yu-kun.Physiological and Biochemical Changes During Regeneration of the Lilium longiflorum Thunb[J].Northern Horticulture,2011(1):149-152. |
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| Authors: | CHEN Li-jing SUN Chun-yu MA Hui TAO Cheng-guang RUAN Yan-ye LI Tian-lai WANG Yu-kun |
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| Institution: | CHEN Li-jing1,SUN Chun-yu1,MA Hui1,TAO Cheng-guang2,RUAN Yan-ye3,LI Tian-lai1,WANG Yu-kun1(1.Bioscience and Technology Institute and Key Laboratory of Protected Horticulture,Ministry of Education,Shenyang Agricultural University,Shenyang,Liaoning 110161,2.The Liaoning Academy of Agricultural Sciences,3.Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081) |
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| Abstract: | With new varieties of the Lilium longiflorum Thunb ShenXiang1 as the experimental material,determined and analyzed the effects of hormones on physiological and biochemical variations of the Lilium longiflorum Thunb,on MS medium added 6-BA 0.5 mg/L+NAA 0.1 mg/L and one without hormone.The results showed that with the hormome treatment of regenerated seedings of the Lilium longiflorum Thunb,the increasing of soluble protein and non-soluble protein content was much bigger.Proper hormome treatment could induce ... |
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| Keywords: | Lilium longiflorum Thunb bulb hormones regeneration physiological and biochemical changes |
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