| 钼蓝比色法测定沙田柚果肉中还原型维生素C含量的研究 |
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| 引用本文： | 刘绍俊,牛英,刘冰浩,陈国平.钼蓝比色法测定沙田柚果肉中还原型维生素C含量的研究[J].北方园艺,2011(1):8-12. |
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| 作者姓名： | 刘绍俊 牛英 刘冰浩 陈国平 |
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| 作者单位： | 广西师范大学,生命科学学院,广西,桂林,541004;广西柑桔研究所,广西,桂林,541004 |
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| 基金项目： | 公益性行业(农业)科研专项经费资助项目(nyhyzx07-023); 广西科学基金资助项目(桂科自0991188) |
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| 摘 要： | 以沙田柚果肉和VC标准液为试材,探讨钼蓝比色法测定沙田柚果肉最佳条件。结果表明:测定波长为700 nm,3%偏磷酸-醋酸用量为1.5 mL,5%硫酸和5%钼酸铵用量均为2 mL,30℃水浴显色20 min,取出自然冷却至室温,再放置1 h后测定。该方法数据的稳定性和准确性好,适合少量和批量样品的测定。试验测得沙田柚果肉中还原型维生素C平均含量为114.63 mg/100g。
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| 关 键 词： | 沙田柚 钼蓝比色法 还原型维生素C 波长 |
Study on Determination of the Content of Reduction-type VC in Chinese Grapefruit's Sarcocarp with Phosphomolybdate-blue Spectrophotometry |
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| LIU Shao-jun,NIU Ying,LIU Bing-hao,CHEN Guo-ping.Study on Determination of the Content of Reduction-type VC in Chinese Grapefruit's Sarcocarp with Phosphomolybdate-blue Spectrophotometry[J].Northern Horticulture,2011(1):8-12. |
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| Authors: | LIU Shao-jun NIU Ying LIU Bing-hao CHEN Guo-ping |
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| Institution: | LIU Shao-jun1,NIU Ying2,LIU Bing-hao2,CHEN Guo-ping2(1.College of Life Science,Guangxi Normal University,Guilin,Guangxi 541004,2.Guangxi Citrus Research Institute,Guangxi 541004) |
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| Abstract: | Chinese grapefruit’s sarcocarp and VC standard liquid as test materils,phosphomolybdate-blue spectrophotometry was established for the best determination of the content of reduction-type VC.The results showed that optimal measuring wavelengths was 700 nm and the dosage of 3% metaphosphoric acid-acetic acid,5 % sulfuric acid,5% molybdate-ammonium in 25 mL reaction system were 1.5 mL,2 mL,2 mL,respectively.The reaction system,water-bathed at 30℃ for 20 minutes and then placed in room temperature for 1 hour,was in stable state.The method was stable,accurate and suitable for the determination of small-amount and batch samples.In this study,the average content of reduction-type VC in Chinese grapefruit’s sarcocarp was 114.63 mg/100g. |
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| Keywords: | Chinese grapefruit molybdenum blue colorimetry reduction-type VC wavelength |
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