杂交兰种间及杂交后代同工酶活性和蛋白质电泳测定与分析

试验测定了杂交兰亲本及其杂交F1代的POD、SOD、CAT的酶活性,并分析了EST和蛋白质的电泳图谱。结果表明:亲本及杂交F1代POD、SOD、CAT同工酶活性,母本的酶活性低于父本;F1代株系中POD、CAT的差异比较明显,SOD的差异较小。由EST电泳酶谱可知母本有3条明显的酶带,父本有2条酶带,F1代出现了丰富的变异,具有亲本酶带特征的F1代株系约占33.3%。通过亲本及杂交F1代蛋白质SDS-PAGE电泳表明,父本比母本多6条较弱的酶带,F1代中比母本多2条较弱谱带的占15.8%;其余的与母本相同。

Determined and Analyzed of Enzymatic Activity and Protein Electrophoretogram in Hybrid Orchids and Their Hybrid Generation

The enzymatic activity of POD,SOD and CAT in hybrid orchids and their hybrid F1 generation were determined,the electrophoretogram of EST and protein were analyzed.The results showed that the POD、SOD and CAT enzymatic activity of the female parents was lower than that of their male parents among parents and their hybrid F1 generation.Among F1 generation,there was significant difference in POD and CAT,while there was no significant difference in SOD.From the electrophoretogram of EST,it could been seen that female parents had three obvious enzymatic bands and male parents had two.At the same time,abundant variations had been seen in hybrid F1 generation.The hybrid F1,which had their parents’ features of the enzymatic bands,account for 33.3%.Protein SDS-PAGE’ electrophoresis of parents and their hybrid F1 showed that male parents had six more weak bands than female ones.15.8% hybrid F1 generation had two more weak bands than female ones,and the rest of them had the equal number to their female parents.