| 重组毕赤酵母摇瓶发酵产木聚糖酶条件优化 |
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| 引用本文： | 周晨妍,张金华,王煜蘅,赵文玺,丰慧根.重组毕赤酵母摇瓶发酵产木聚糖酶条件优化[J].安徽农业科学,2010,38(22):11696-11697,11700. |
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| 作者姓名： | 周晨妍 张金华 王煜蘅 赵文玺 丰慧根 |
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| 作者单位： | 新乡医学院,河南省医学遗传学与分子靶向药物高校重点实验室培育基地,河南新乡,453003;新乡医学院护理学院,河南新乡,453003 |
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| 摘 要： | 目的]在摇瓶发酵条件下,研究甲醇诱导毕赤酵母工程菌K3产木聚糖酶表达规律。方法]以重组木聚糖酶酶活为评价指标,采用单因素试验和L9（34）正交试验考察摇瓶发酵条件下pH值、接种时间、诱导剂浓度、诱导时间对酶活的影响。结果]各因素影响程度依次为：pH值〉接种时间〉诱导时间〉甲醇浓度,最优表达条件为：pH值5.3,接种时间19h,甲醇诱导浓度1.25%,诱导时间192h,在此条件下菌株产酶酶活可达589.16IU/ml。结论]该研究为木聚糖酶的工业化生产和应用提供依据。
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| 关 键 词： | 木聚糖酶 毕赤酵母 甲醇诱导 发酵 |
Optimization of Flask Liquid Fermentation for Xylanase Producing by Recombinant Pichia pastoris |
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| ZHOU Chen-yan et al.Optimization of Flask Liquid Fermentation for Xylanase Producing by Recombinant Pichia pastoris[J].Journal of Anhui Agricultural Sciences,2010,38(22):11696-11697,11700. |
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| Authors: | ZHOU Chen-yan |
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| Institution: | ZHOU Chen-yan et al (Key Laboratory of Medical Genetics , Molecular Targeting Drugs,Henan Province,Xinxiang Medical University,Xinxiang,Henan 453003) |
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| Abstract: | Objective] In order to find out the best fermentation conditions for xylanase production by Pichia pastoris K3,using methanol as the inducer,the influence of different factors on xylanase expression were studied.Method]With the xylanase activity of recombinant xylanase as the evaluation index,the effects of of culture conditions such as pH,inoculation time,methanol concentration and induction time on the expression of the recombinant xylanase of Pichia pastoris K3 were investigated by using single factor ... |
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| Keywords: | Xylanase Pichia pastoris Methanol induction Fermentation |
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