大豆对灰斑病菌15号小种的抗病基因定位及标记检测

为明确大豆对灰斑病菌15号小种的抗性位点,以大豆抗病品种垦丰16、感病品种绥农10及其杂交F₂、F₃代群体为试验材料,在接种鉴定的基础上,运用SSR标记技术及分离群体组群分析法(BSA法)对垦丰16抗病基因进行了定位,并应用108份大豆新品系对标记进行了符合性检测。结果表明,垦丰16对15号小种的抗性受1对显性基因控制,抗病基因位于大豆染色体组的J连锁群上,将该基因定名为Rcs15。用Mapmaker/Exp 3.0 b进行连锁分析,获得了5个与抗病基因紧密连锁的SSR标记:Satt 529、Satt 431、Sat_151、Satt 547和Sat_224,标记与抗病基因间的排列顺序和遗传距离为Sat_151-10.7 cM-Satt 529-18.5 cM- Rcs15-6.7 cM-Satt 547-7.8 cM-Sat_224-10.7 cM-Satt 431。标记符合性检测结果显示,Satt 547和Sat_224的检测准确率达到85%以上,可用于分子标记辅助选择育种和抗源筛选。

Identification and mapping of the Cercospora sojina race 15 resistance gene in soybean

The main objective of this study was to identify and map the Cercospora sojina race 15 resistance gene in soybean Kenfeng 16 with simple sequence repeat (SSR) markers. Soybean plants of Suinong 10 (susceptible), Kenfeng 16 (resistant) and F₂ and F₃ populations derived from a cross of these two cultivars were screened for the inheritance analysis of C.sojina race 15 resistance. SSR markers linked to the C.sojina race 15 resistance gene were identified using bulked segregation analysis. Furthermore, 108 soybean breeding lines were analyzed for the marker efficiency. The results showed that resistance to C.sojina race 15 in Kenfeng 16 was controlled by one pair of dominant genes, which located on linkage group J of soybean genome and was named Rcs15. The result of linkage analysis by using Mapmaker/Exp 3.0 b software showed that five SSR markers, Satt 529, Satt 431, Sat_151, Satt 547 and Sat_224, were tightly linked to the resistance gene. The order and genetic distance of the markers in the resistance gene locus were Sat_151-10.7 cM-Satt 529-18.5 cM-Rcs15- 6.7 cM-Satt 547-7.8 cM-Sat_224-10.7 cM-Satt 431. Conformance analysis showed that the screening efficiency of markers Satt 547 and Sat_224 was over 85%. These two SSR markers are useful for molecular marker assisted selection for C.sojina resistance and soybean breeding program.