稻瘟病菌MGG14093基因功能的初步研究

根据之前的基因芯片分析,发现在稻瘟病菌侵染水稻的过程中,许多基因被诱导表达。MGG14093为其中一个推测为编码分泌蛋白的未知功能基因,在病菌侵染过程中该基因表达量上调2.906。利用生物信息学在线软件对该基因编码蛋白的特性进行初步分析,并通过RT-PCR扩增进一步分析该基因在稻瘟病菌侵染水稻过程中的表达动态,利用基因敲除和过量表达技术对该基因的功能进行初步研究。结果表明:该基因编码蛋白为定位于胞外的分泌蛋白,没有已知的蛋白结构域,但可能存在几处氨基酸活性位点。MGG14093基因敲除和过量表达后,对稻瘟病菌的营养生长、产孢及附着胞分化没有显著的影响,但该基因过量表达后导致病菌的致病力下降,表明该基因可能参与病菌的致病过程。本研究结果为进一步揭示MGG14093基因的生物学功能奠定了基础。

Primary Functional Analysis of MGG14093 in Magnaporthe oryzae

According to our microarray analysis results, a large amount of genes were induced during the rice infection process of the rice blast fungus Magnaporthe oryzae, and among which MGG14093 was upregulated 2.906 times, and predicted to be a secreted protein with unknown function. In this study, the protein properties of MGG14093 was predicatively parsed using diverse online bioinformatics softwares, the expression pattern during the rice infection process was further proved by RT-PCR, and the function was studied through the strategies of target gene replacement and overexpression. Our results showed that no known functional domain was found in MGG14093, but there might be some active sites. Neither null nor overexpression mutant of MGG14093 could obviously affect the vegetative growth, conidiation and appressorial development in M. oryzae, while the pathogenicity was reduced after its overexpression, which suggested that it could be involved in the pathogenesis. Our study established the foundation for the further revelation of the biological function of MGG14093.