| 羊传染性脓疱病毒吉林株主要囊膜蛋白B2L基因的克隆与序列分析 |
| |
| 引用本文： | 程荣华,刘云志,毛文智,孙健,邵洪泽,呼延含蓉,杨金生.羊传染性脓疱病毒吉林株主要囊膜蛋白B2L基因的克隆与序列分析[J].吉林畜牧兽医,2010,31(2):5-7. |
| |
| 作者姓名： | 程荣华 刘云志 毛文智 孙健 邵洪泽 呼延含蓉 杨金生 |
| |
| 作者单位： | 1. 吉林省畜牧兽医科学研究院,吉林长春,130062;吉林农业大学,吉林长春,130118
2. 吉林省畜牧兽医科学研究院,吉林长春,130062 |
| |
| 基金项目： | 吉林省科技发展计划资助项目:20080106 |
| |
| 摘 要： | 参照Genbank自行设计2对引物,对羊传染性脓疱病毒JS04株病毒DNA进行两次扩增,扩增产物与pMD19-T连接后,热转化至E.coli Competent Cells DH5中,涂布平板,37℃过夜培养,挑选阳性菌落殖菌,提取质粒,对质粒上产物基因测序,序列大小1137bp,并导出其相关的氨基酸序列。序列分析表明,该毒株B2L基因与ORF-NZ2、Shanhjahnpur82/04等毒株的核苷酸序列同源性为97.8%～99.5%,氨基酸序列同源性为97.6%～99.5%。B2L基因的系统发育进化树结果表明,不同地区分离毒株的B2L基因差异不大。
|
| 关 键 词： | 羊传染性脓胞病毒 B2L基因 克隆 序列分析 |
Cloning and Sequence Analysis of B2L Gene of Orf Virus Isolated from Jilin Province |
| |
| CHENG Rong-hua,LIU Yun-zhi,MAO Wen-zhi,SUN Jian,SHAO Hong-ze,HUYAN Han-Rong,YANG Jin-sheng.Cloning and Sequence Analysis of B2L Gene of Orf Virus Isolated from Jilin Province[J].Jilin Animal Science and Veterinary Medicine,2010,31(2):5-7. |
| |
| Authors: | CHENG Rong-hua LIU Yun-zhi MAO Wen-zhi SUN Jian SHAO Hong-ze HUYAN Han-Rong YANG Jin-sheng |
| |
| Institution: | 1.Jilin Academy of Animal Science & Veterinary Medicine;Changchun Jilin 130062;2.Jilin Agricultural University;Changchun Jilin 130118 |
| |
| Abstract: | According to the DNA sequence of Orf virus which was published on Genbank,two pairs of primers have been designed to amplify B2L gene of JS04 by PCR.The amplified production was combined with pMD19-T vector,then was transformed into DH5.Positive clones identified correctly were sequenced.The length of B2L gene was 1137bp,and the corresponding amino acid sequence was conducted.Sequence analysis showed that the B2L gene homology among ORFNZ2,Shanhjahnpur82/04 strains was 97.8%～ 99.5%.The amino acid sequence homology was 97.6%～ 99.5%.The analysis result of phylogenetic tree showed that the B2L gene. |
| |
| Keywords: | Orf virus B2L gene cloning sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
