添加沼液的BG11营养液微藻培养试验

利用沼液培育能源微藻,有利于废水处置的同时还能够有效降低微藻生产成本。该文研究了S496、1067、1069、C31、Y7、Y3及W 7株微藻在混有体积分数10%沼液的培养液中的比生长速率、培养液pH值和营养物的变化规律。结果表明,除Y7外,其它6株微藻均能迅速适应沼液营养液,迅速进入对数期生长。在未调节培养液pH值条件下,沼液培养液的pH值在培养的前2～4 d均有所下降,从9.20～9.32下降至8.73～9.09,之后才开始恢复并上升。在pH值下降时,藻的比生长速率有不断增大的趋势。培养12 d后,上述7株微藻在培养液中干质量累积量在0.310～0.607 g/L之间;培养过程中,NH4-N、Mg2+、SO42-、Mn、F-、Fe的浓度都大幅下降,NH4-N、Mn、Fe几乎被完全消耗;Mg2+降幅为11.47%～87.73%;SO42-为37.30%～62.70%;F-为18.18%～54.55%。该文为利用沼液培养能源微藻提供依据。

Experiment on microalgae cultivation in BG11 nutrient solution adding biogas slurry

The way of using biogas slurry to cultivate energy microalgae is favorable to wastewater disposal and can lower the cost of the algae production effectively.7 strains of microalgae S496,1067,1069,C31,Y7,Y3 and W were added to be mixed with nutrient solution(volume ratio of biogas slurry and BG11 was 1:9),and then changes of the growth rate,pH value and nutrients of nutrient solution were studied in this paper.The results showed that all other 6 trains could adapt to culture solutions immediately and came into logarithm growth phase expect Y7.Under the conditions of unregulated medium pH value,pH value of these culture solutions all decreased from 9.20-9.32 to 8.73-9.09 in the first 2-4 days and then started to rise.When pH value declined,growth rate of microalgae increased constantly.When algae were cultivated after 12 days,the final concentrations of algae in culture medium were between 0.310-0.607 g/L.During culture process,the concentrations of NH4-N,Mg,SO42-,Mn,F-and Fe all dropped significantly;NH4-N、Mn、Fe were fully absorbed;Mg2+ dropped 11.47%-87.73%;SO42-dropped 37.30%-62.70%;F-dropped 18.18%-54.55%.This research provides scientific basis for cultivating microalgae in mixing biogas slurry.