肝癌细胞系中Runx3基因表达及启动子区异常甲基化分析

目的：通过检测6种肝癌细胞中Runx3基因异常甲基化状态及表达情况，探讨药物5-aza-2＇-deoxycytidine（decitabine）激活Runx3基因重新表达的能力及对肝癌细胞生长的影响。方法：采用DNA甲基化特异性PCR（MSP）技术分别对6种肝细胞癌细胞系Runx3基因启动子区域甲基化状态进行检测，同时采用逆转录一聚合酶链反应（RT—PCR）检测5种肝癌细胞中Runx3的表达情况及药物5-aza-2＇-deoxycytidine处理其中3种肝癌细胞前后Runx3表达变化。结果：6种肝癌细胞系中，有3种细胞Runx3基因启动子区域存在甲基化异常，药物5-aza-2＇-deoxycytidine处理3种肝癌细胞后，Runx3基因明显表达或表达活性增强。结论：启动子区异常甲基化是导致Runx3基因失活的主要原因之一，与肝细胞癌发生早期密切相关，可作为肝细胞癌早期诊断的分子标记物及分子治疗的靶点。

Expression and Hypermethylation of Runx3 gene in Promoter Region with Hepatocellular Carcinoma Cell Lines

Objective:To detect the status of promoter hypermethylation and expression of Runx3 gene in six hepatocellular carcinoma(HCC) cell lines and investigate the effect of 5-aza-2'-deoxycytidine(decitabine) on cell growth and the ability of reactivation of Runx3 gene.Methods:The methylation status of Runx3 was examined by methylation-specific polymerase chain reaction(MSP).Meanwhile,RT-PCR was used to detect expression of Runx3 gene in six HCC cell lines and three cell lines that were treated with 5-aza-2'-deoxy...