不同灭菌条件对蓝莓组培影响试验 |
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引用本文: | 李丽容,金开正,赖联森.不同灭菌条件对蓝莓组培影响试验[J].中国园艺文摘,2012,28(2):10-11. |
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作者姓名: | 李丽容 金开正 赖联森 |
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作者单位: | 1. 杭州万向职业技术学院,浙江杭州,310023 2. 浙江益康科技有限公司,浙江长兴,313100 |
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摘 要: | 以蓝莓茎段为外植体,比较不同灭菌条件对蓝莓成活率的影响,结果表明:灭菌方法为70%酒精30s+0.1%升汞、灭菌时间为6~8min时,蓝莓茎段外植体成活率最高,其次是70%酒精30s+2%次氯酸钠、灭菌时间为8~10min。
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关 键 词: | 蓝莓 外植体 灭菌条件 |
The influence of sterilization conditions on blueberry tissue culture |
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Authors: | LI Li-rong JIN kai-zheng LAI Lian-sen |
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Institution: | LI Li-rong, JIN kai-zheng, LAI Lian-sen |
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Abstract: | Taking blueberry stems as explants, the blueberry survival rates under different sterilization conditions were compared. The results show that the sterilization condition for highest explant survival rate was 70% alcohol for 30 s plus 0.1% sublimate for 6 to 8 minutes. The sterilization condition of 70% alcohol for 30 s plus 2% sodium hypochlorite for 8-10 minutes took second place. |
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Keywords: | Explants Sterilization conditions |
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