持续低温对彩叶树木枝条内渗透物质影响的研究

试验研究了4种彩叶树木在-20℃低温持续胁迫下离体枝条内渗透物质含量变化规律,为揭示离体枝条生理特性和判断树木抗寒性提供理论依据。试验以处于深休眠的紫叶矮樱,美国红栌,鸡爪槭,元宝枫为试材,离体枝条在保水条件下,使用冰箱提供-20℃低温胁迫,3次重复。结果表明:4种彩叶树木枝条内可溶性糖含量呈现出升高的变化,升高幅度分别达到93.56%,162.45%,114.95%,57.19%;美国红栌枝条内可溶性蛋白表现为先升高后降低的变化,其余3个树种表现出一直升高的变化,试验末期含量显著高于第3天;紫叶矮樱和鸡爪槭试验期间游离脯氨酸含量无显著差异,美国红栌和元宝枫第15天含量显著高于第3天。综合分析认为,彩叶树木离体枝条在-20℃低温胁迫下仍然具有一定的生理活性,紫叶矮樱抗寒性略高于其他3个树种。

Study on Effects of Coleus Trees Branches of Material of Osmotic in Lasting Low Temperature

To study in continues -20 ℃ low temperature stress, branches of trees within the garden in vitro permeability changes of substance, It is to provide a theoretical basis for the deep sleep physiological changes in vitro the branches and determine the cold hardiness of trees. Experiment with in the deep dormancy of Prunus× Cistena Pissardii, Cotinus coggygria ‘ Atropurpureus’, Acer Palmatum, Acer truncatum Bunge for the tested material, branches in water-in vitro conditions, -20 ℃ low--temperature stress to provide use the refrigerator, repeat 3 times. The results showed that： soluble sugar content showing a higher changes in four kinds of coleus trees branches and respectively increased rate as 93. 56%, 162.45%, 114. 95%, 5Z. 19%; in Cotinus coggygria ‘ Atropurpureus’ branches, soluble protein changes expression as first increased and then decreased and other three species has been showed increased changes and when the test was end significantly higher than the first 3 days; the free proline was no significant difference in Prunus× Cistena Pissardii and Acer Palmatum branches and the 15th days was significantly higher than the 3th days. Comprehensive analysis that colorful tree branches away from the body under cold stress at --20 ℃ still has some physical activity and cold resistance Prunus× Cistena Pissardii slightly higher than the other three species.