| 逆转录病毒载体及其在动物病毒病的研究进展 |
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| 引用本文： | 吕素芳,郭广君,肖跃强,沈志强.逆转录病毒载体及其在动物病毒病的研究进展[J].动物医学进展,2011,32(2):86-90. |
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| 作者姓名： | 吕素芳 郭广君 肖跃强 沈志强 |
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| 作者单位： | 山东省滨州畜牧兽医研究院;山东绿都生物科技有限公司; |
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| 摘 要： | 逆转录病毒系统由于其病毒的高感染效率,在RNA干扰中的应用越来越多,在对基因功能的研究中起了很大的作用.逆转录病毒表达系统是一种新的重组蛋白高效表达系统,由逆转录病毒载体、包膜蛋白载体和包装细胞系组成.逆转录病毒载体具有基因组结构简单,便于基因操作、转染效率高、容量较大、宿主范围广、易于分离等优点,在外源基因表达、基因...
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| 关 键 词： | 逆转录病毒表达系统 分子生物学特性 动物病毒病 |
Progress on Retroviral Vectors in Animal Virosis |
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| LU Su-fang,GUO Guang-jun,XIAO Yue-qiang,SHEN Zhi-qiang.Progress on Retroviral Vectors in Animal Virosis[J].Progress In Veterinary Medicine,2011,32(2):86-90. |
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| Authors: | LU Su-fang GUO Guang-jun XIAO Yue-qiang SHEN Zhi-qiang |
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| Institution: | 1,2(1.Shandong Binzhou Animal Science & Veterinary Medicine Insitute,Binzhou,Shandong,256600,China;2.Shandong Lvdu Bio-industry Company,LTD.,Binzhou,Shandong,256600,China) |
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| Abstract: | RNA interference is a high popular research topic in recent years.The retrovirus expression system has played an important role in the study of gene function because of its high efficiency.The retrovirus expression system which composed of retrovirus vectors,envelope protein vectors and packaging cell lines is a new expression system of recombinant proteins.Retrovirus vectors exhibit unique properties due to their simple genome structure,ease of genetic manipulation,transfection efficiency,larger capacity,b... |
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| Keywords: | retrovirus expression system molecular biological characteristic animal virosis |
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