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朱鹮低致病性禽流感病毒的分离与鉴定
引用本文:李六斤,毛峰峰,师长宏,张海,赵勇,张彩勤,白冰. 朱鹮低致病性禽流感病毒的分离与鉴定[J]. 动物医学进展, 2011, 32(2): 42-44
作者姓名:李六斤  毛峰峰  师长宏  张海  赵勇  张彩勤  白冰
作者单位:第四军医大学实验动物中心;
基金项目:陕西省自然科学研究计划项目(2004C1-25)
摘    要:为了掌握朱鹮禽流感病毒流行情况,利用鸡胚分离方法从病死朱鹮脑组织标本中分离出禽流感病毒毒株,并将所分离的病毒进行血凝试验、血凝抑制试验、鸡胚最小致死量平均死亡时间(MDT)、半数致死量(LD50)及1日龄鸡脑内接种致病指数(ICPI)试验.结果表明,所分离的病毒均能够凝集鸡、肉鸭、绵羊、山羊、猪、人、兔等的红细胞,且这...

关 键 词:朱鹮  禽流感病毒  分离鉴定

Isolation and Identification of LPAIV from Crested Ibis
LI Liu-jin,MAO Feng-feng,SHI Chang-hong,ZHANG Hai,ZHAO Yong,ZHANG Cai-qin,BAI Bing. Isolation and Identification of LPAIV from Crested Ibis[J]. Progress In Veterinary Medicine, 2011, 32(2): 42-44
Authors:LI Liu-jin  MAO Feng-feng  SHI Chang-hong  ZHANG Hai  ZHAO Yong  ZHANG Cai-qin  BAI Bing
Affiliation:LI Liu-jin,MAO Feng-feng,SHI Chang-hong,ZHANG Hai,ZHAO Yong,ZHANG Cai-qin,BAI Bing(Laboratory Animal Center,The Fourth Military Medical University,Xi'an,Shaanxi,710032,China)
Abstract:To isolate and identify avian influenza virus in crested ibis(Nipponian nippon),specimens were collected from dead crested ibis.The suspension of brain tissue of crested ibis was used to inoculate SPF chicken embryo,then the AIV was isolated.Hemagglutination test(HA),hemagglutination inhibition(HI),the death time of chicken embryos(MDT),median lethal dosage(LD50) and the intracerebral pathogenicity index(ICPI) tests were carried out respectively to identify the serotype of AIV.The strain could agglutinate t...
Keywords:crested ibis  Avian influenza virus  isolation and identification  
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