| 己烯雌酚免疫抗原的合成与鉴定 |
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| 引用本文： | 李利东,宓晓黎,黄丽俊,袁建兴,杜姝莲. 己烯雌酚免疫抗原的合成与鉴定[J]. 动物医学进展, 2011, 32(2): 29-32 |
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| 作者姓名： | 李利东 宓晓黎 黄丽俊 袁建兴 杜姝莲 |
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| 作者单位： | 江苏省微生物研究所有限责任公司; |
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| 基金项目： | 国家863高新技术发展计划课题(2008AA10Z425); 江苏省科技支撑计划(BE2008631); 无锡市科技支撑计划(CSE00804) |
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| 摘 要： | 通过重氮化法引入羧基,再用碳二亚胺法偶联BSA获得己烯雌酚(DES)免疫抗原.经紫外光谱、电泳图谱分析确证获得DES-BSA偶联物.间接酶联免疫法分析证明其免疫鼠所获得的抗体与己烯雌酚有良好的特异性.
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| 关 键 词： | 己烯雌酚 人工抗原 重氮化 碳二亚胺法 |
Synthesis and Identification of Artificial Antigens of Diethylstibestrol |
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| LI Li-dong,MI Xiao-li,HUANG Li-jun,YUAN Jian-xing,DU Shu-lian. Synthesis and Identification of Artificial Antigens of Diethylstibestrol[J]. Progress In Veterinary Medicine, 2011, 32(2): 29-32 |
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| Authors: | LI Li-dong MI Xiao-li HUANG Li-jun YUAN Jian-xing DU Shu-lian |
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| Affiliation: | LI Li-dong,MI Xiao-li,HUANG Li-jun,YUAN Jian-xing,DU Shu-lian(Jiangsu Institute of Microbiology Co.Ltd.,Wuxi,Jiangsu,214063,China) |
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| Abstract: | The immunogen DES-BSA(diethylstibestrol-bovine serum albumin) were synthesized by the carbodilimide method through introduction of a carboxyl group into diethylstilbestrol by diazotization method.The results from UV,SDS-PAGE and ELISA showed that DES-BSA were conjugated successfully.The indirect ELISA results showed that antibodies derived from mice were specific to diethylstilbestrol. |
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| Keywords: | diethylstilbestrol artificial antigen diazotization carbodiimide method |
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