几种金属离子对沼泽红假单胞菌2-8生长和亚硝酸盐消除的影响

在培养基中添加一定浓度金属离子,研究了不同浓度铜离子（Cu2＋）、镁离子（Mg2＋）、锌离子（Zn2＋）、铁离子（Fe3＋）和锰离子（Mn2＋）对沼泽红假单胞菌（Rhodopseudom onas palustris）2-8株生长和亚硝酸盐消除能力的影响。结果发现,1×10-7mol.L-1Cu2＋刺激菌株生长和亚硝酸盐消除,1×10-4mol.L-1Cu2＋抑制生长和亚硝酸盐消除,1×10-6mol.L-1和1×10-5mol.L-1的Cu2＋抑制生长,但刺激亚硝酸盐消除;1×10-4mol.L-1Mg2＋刺激菌株生长和亚硝酸盐消除;不同浓度Zn2＋对菌体生长影响不显著,但浓度为1×10-6mol.L-1和1×10-7mol.L-1时刺激亚硝酸盐消除,浓度大于1×10-6mol.L-1时抑制亚硝酸盐消除;Fe3＋促进生长,浓度越高生长越好,对菌株亚硝酸盐消除的影响则刚好相反;不同浓度Mn2＋均抑制菌株生长,1×10-6～1×10-3mol.L-1Mn2＋抑制亚硝酸盐消除。受测定的5个金属离子中除1×10-4mol.L-1Cu2＋对菌株亚硝酸盐消除能力的抑制较强外,其他离子的抑制会随时间推移逐渐消失。

Influence of several metal ions on growth and nitrite removal of Rhodopseudomonas palustris strain 2-8

We supplemented different concentrations of Cu2＋,Mg2＋,Zn2＋,Fe3＋,Mn2＋ to the substrate to study their influence on the growth and nitrite removal of Rhodopseudomonas palustris strain 2-8.It is found that 1×10-7 mol·L-1 Cu2＋ improves but 1×10-4 mol·L-1 Cu2＋ inhibits the growth and nitrite removal of strain 2-8;1×10-6 mol·L-1 and 1×10-5 mol·L-1 Cu2＋ inhibit the growth but improve the nitrite removal.1×10-4 mol·L-1 Mg2＋ improves the growth and nitrite removal.Zn2＋ has no significant influence on the growth but low concentration（1×10-6mol·L-1 and 1×10-7mol·L-1） of Zn2＋ improves the nitrite removal,while high concentration（1×10-6 mol·L-1） is the opposite.The higher the concentration of Fe3＋ is,the better the growth is but the worse the nitrite removal is.Mn2＋ inhibits the growth and 1×10-6～1×10-3 mol·L-1 Mn2＋ inhibits the nitirte removal.Except 1×10-4 mol·L-1 Cu2＋ which inhibits nitrite removal greatly,the inhibition of the other metal ions becomes weaker with time.