| 鸽新城疫病毒弱毒株的分离与鉴定 |
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| 引用本文： | 刘健,张维谊,周锦萍,卢军.鸽新城疫病毒弱毒株的分离与鉴定[J].动物医学进展,2007,28(10):48-50. |
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| 作者姓名： | 刘健 张维谊 周锦萍 卢军 |
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| 作者单位： | 上海市畜牧兽医站,上海,201103;上海市畜牧兽医站,上海,201103;上海市畜牧兽医站,上海,201103;上海市畜牧兽医站,上海,201103 |
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| 摘 要： | 对某鸽场送检的6只通过病理剖检疑似新城疫(ND)的病鸽进行实验室诊断,包括细菌分离、新城疫病毒荧光RT-PCR检测、SPF鸡胚分离与鉴定,并测定其鸡胚分离物F3代EID50和ICPI.结果为内脏病料新城疫病毒荧光RT-PCR检测为阳性;SPF鸡胚分离到一株有血凝活性的病毒,经HA和HI鉴定为NDV,其F3代血凝价为1∶26,EID50为107.63,ICPI为1.19.根据病鸽的临床症状、鸡胚分离物EID50和IC-PI,确定该鸽新城疫病毒分离株为弱毒株.
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| 关 键 词： | 新城疫病毒 分离 鉴定 鸽 |
| 文章编号： | 1007-5038（2007）10-0048-03 |
| 修稿时间： | 2007-06-04 |
Isolation and Identification of a Low Rirulent Newcastle disease virus Strain in Pigeon |
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| LIU Jian,ZHANG Wei-yi,ZHOU Jin-ping,LU Jun.Isolation and Identification of a Low Rirulent Newcastle disease virus Strain in Pigeon[J].Progress In Veterinary Medicine,2007,28(10):48-50. |
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| Authors: | LIU Jian ZHANG Wei-yi ZHOU Jin-ping LU Jun |
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| Institution: | Shanghai Animal Husbandry and Veterinary Station, Shanghai, 201103, China |
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| Abstract: | 6 pigeons from a pigeon farm in Shanghai were diagnosed by laboratory methods,including: clinical diagnosis;bacterial isolation;NDV fluorescence RT-PCR detection;SPF chicken embryo isolation and identification.The HA and HI tests indicated that the isolated strain was NDV.The 50% embryo infectious dose(EID50) and intracerabral pathogenicity index(ICPI) of SPF chicken embryo allantoid liquid were 107.63 and 1.19,respectively.According to the clinical symptom,EID50,ICPI,the isolated pigeon NDV is a low virulent strain. |
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| Keywords: | NDV isolation identification pigeon |
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