无刺枸骨果实化学成分及其抗氧化和抑菌活性研究

[目的 ]探究无刺枸骨果实的化学成分及功能,为种质资源的开发利用提供基础。[方法 ]利用正丁醇、乙酸乙酯和石油醚等萃取剂对无刺枸骨果实成分进行萃取,并采用2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)法、1,1-二苯基-2-三硝基苯肼(DPPH)法和滤纸片抑菌法测定其萃取物的抗氧化与抑菌活性;结合气相色谱-质谱联用(GC-MS)技术对正丁醇萃取物的化学组分进行鉴定。[结果 ]无刺枸骨果实甲醇粗提取物的正丁醇、乙酸乙酯和石油醚萃取物对ABTS和DPPH自由基均有明显的清除作用,且清除率与萃取物浓度间呈线性正相关。抑菌试验结果表明:正丁醇提取物对于所有菌株均具有极显著的抑菌作用,且是3种萃取剂中抑菌作用最强的;而乙酸乙酯萃取物对于枯草芽孢杆菌、鼠伤寒沙门氏菌和变形杆菌也具有极显著抑菌作用;石油醚萃取物对枯草芽孢杆菌、鼠伤寒沙门氏菌和变形杆菌抑制作用显著。结合GC-MS与UPLC-QTOF/MS技术,本文首次证实无刺枸骨果实含有抗氧化与抑菌活性的药用成分——绿原酸。[结论 ]本研究首次采用多种萃取剂对无刺枸骨果实成分进行萃取和鉴定,并通过试验证明其萃取物具有显著的抗氧化和...

Study on Constituents of Ilex cornuta var. fortunei Fruit and Their Antioxidant and Bacteriostatic Activity

Objective To explore the chemical composition and function of the fruit of Ilex cornuta var. fortunei for the purpose of development and utilization. Method Firstly, 1-butanol, ethyl acetate and petroleum ether were used to extract the active components from the fruit of I. cornuta var. fortunei. Then the free radical scavenging assays against 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazine (DPPH) coupled with the filter paper slice bacteriostasis were used to evaluate their antioxidant and bacteriostatic activities. Meanwhile, the chemical components of 1-butanol extraction were identified by gas chromatography-mass spectrometry (GC-MS). Result The 1-butanol, ethyl acetate and petroleum ether extracts from the crude methanol extract of the fruits of I. cornuta var. fortunei had significant scavenging effects on ABTS and DPPH free radicals, and the scavenging rate was positively correlated with the extract concentration. The results of antibacterial test showed that the 1-butanol extraction had the best antibacterial effect among all strains tested, and had the strongest bacteriostatic effect among the three extractants. The ethyl acetate extraction and the petroleum ether extract had the best antibacterial effect on Bacillus subtilis, Salmonella typhimurium, and Proteusbacillus vulgaris. Combining with GC-MS and UPLC-Q-TOF/MS technology, it was reported for the first time that the fruits of I. cornuta var. fortunei contains chlorogenic acid, a medicinal ingredient with antioxidant and antibacterial activity. Conclusion In this study, a variety of extractants were used for the first time to extract and identify the components of the fruits of I. cornuta var. fortunei, and the results show that the extracts have significant antioxidant and antibacterial activities..