| 适于AFLP分析的澳洲坚果DNA提取方法 |
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| 引用本文： | 李惠波,倪书邦,贺熙勇. 适于AFLP分析的澳洲坚果DNA提取方法[J]. 热带农业科技, 2005, 28(1): 1-2,5 |
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| 作者姓名： | 李惠波 倪书邦 贺熙勇 |
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| 作者单位： | 1. 西南农业大学,重庆,北碚,400716
2. 云南省热带作物科学研究所,云南,景洪,666100 |
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| 基金项目： | 云南省科技攻关项目资助(2003NG07) |
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| 摘 要： | 利用SDS法和改良CTAB法对澳洲坚果两个品种的基因组DNA进行提取试验,结果表明:SDS法与改良CTAB法提取的基因组DNA完整性都比较好,但SDS法获得的DNA含有较多杂质,不能被限制性内切酶消化,改良CTAB法提取的基因组DNA可以完全酶切,DNA质量符合AFLP分析的要求。
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| 关 键 词： | 澳洲坚果 DNA提取 改良CTAB法 AFLP |
| 文章编号： | 1672-450X(2005)01-0001-02 |
Genomic DNA Isolation Methods for AFLP Analysis of Macadamia integrifolia |
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| LI Hui-bo,NI Shu-bang,HE Xi-yong. Genomic DNA Isolation Methods for AFLP Analysis of Macadamia integrifolia[J]. Tropical Agricultural Science & Technology, 2005, 28(1): 1-2,5 |
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| Authors: | LI Hui-bo NI Shu-bang HE Xi-yong |
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| Affiliation: | LI Hui-bo1,NI Shu-bang2,HE Xi-yong2 |
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| Abstract: | Genomic DNA of Macadamia integrifolia was extracted by using SDS and the improved CTAB. Results showed that DNA obtained by the improved CTAB had good integrality, and it was so pure as to be completely digested by restriction endonuclease and could be used for AFLP Amplification reaction, but DNA obtained by SDS could not be digested. |
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| Keywords: | Macadamia integrifloia DNA Isolation improved CTAB AFLP |
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