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| 稻瘟病菌突变菌株的分子鉴定 | |
| 引用本文： | 何月秋,Hei LEUNG,唐文华.稻瘟病菌突变菌株的分子鉴定[J].农业生物技术学报,2003,11(1):89-93. |
| 作者姓名： | 何月秋 Hei LEUNG 唐文华 |
| 作者单位： | 1. 云南农业大学植物保护学院,昆明,650201;国际水稻研究所昆虫与植病系,菲律宾马卡提市,3127 2. 国际水稻研究所昆虫与植病系,菲律宾马卡提市,3127 3. 中国农业大学植物保护学院,北京,100094 |
| 基金项目： | 国际水稻研究所的生物多样性持续控制水稻病虫害项目. |
| 摘 要： | 摘要：利用Rep-PCR的两对引物Pot2-1/Pot2-2和PMC1-2/PMC1-3、 177条RAPD引物和MGR586为探针的3种RFLP 分子技术对温室的9个和自然病圃上的5个致病性突变菌株进行分析。3种技术鉴定了温室9个菌株与其起始菌株的DNA指纹基本一致，检测到7个菌株DNA条带缺失1~3条，2个菌株没有变化；分析了病圃上的侵染携带抗病基因Pi-1和Pi-2的5个突变株的起源，依据DNA指纹和致病类型证明后者起源于具有无毒基因Avr-1的菌株，排除了外来菌株的漂移作用，证实了突变是稻瘟病菌(Magnaporthe grisea )进化的主要动力。 |
| 关 键 词： | 关键词：稻瘟病菌 突变菌株 分子鉴定 |
| 修稿时间： | 2002年1月12日 |
Molecular Identification of the Mutant Isolates of Magnaporthe grisea |
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| Hei LEUNG,Robert S.ZEIGLER.Molecular Identification of the Mutant Isolates of Magnaporthe grisea[J].Journal of Agricultural Biotechnology,2003,11(1):89-93. | |
| Authors: | Hei LEUNG Robert SZEIGLER |
| Abstract: | Abstract: Pathogenic mutant isolates 9 from the greenhouse and 5 from the International Rice Research Institute Blast Nursery, respectively, were identified by using molecular techniques with Rep-PCR of the two primer pairs, Pot2-1/Pot2-2 and PMC1-2/PMC1-3, RAPD of 177 primers and RFLP of MGR586. The results indicated that the 9 mutants from greenhouse had almost identical DNA band patterns and their original isolates lost 1~3 bands for 7 mutants, but no changes for the other 2 mutants. The 5 mutants from the nursery, which defeated resistance genes Pi-1 and Pi-2, originated from the isolates with avirulence gene Avr-1 in the field, not from outside by genetic shift based on their pathotypes and DNA band patterns. Therefore, it was concluded that the mutation was a main evolution force for Magnaporthe grisea. |
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