| 高效产纤维素酶菌株ZJW-6发酵条件优化 |
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| 引用本文： | 唐蕊. 高效产纤维素酶菌株ZJW-6发酵条件优化[J]. 湖北农业科学, 2012, 51(6): 1232-1233 |
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| 作者姓名： | 唐蕊 |
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| 作者单位： | 邢台学院生物化学系,河北邢台,054001 |
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| 摘 要： | 在筛选出纤维素酶高产菌株的基础上,对纤维素酶高产菌株ZJW-6采用单因素试验进行不同条件下的液体发酵培养,使用DNS法对发酵后的菌悬液进行酶活力测定从而获得其最优发酵条件.结果表明,菌株ZJW-6产纤维素酶的最优发酵条件是以蛋白胨+(NH4)2SO4为氮源培养基,在30℃、pH 6下振荡培养48 h.
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| 关 键 词： | 纤维素分解菌 发酵条件 纤维素酶 酶活力 |
Research on the Optimum Fermentation Conditions of High-Yield Cellulolytic Enzymes Strain ZJW-6 |
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| TANG Rui. Research on the Optimum Fermentation Conditions of High-Yield Cellulolytic Enzymes Strain ZJW-6[J]. Hubei Agricultural Sciences, 2012, 51(6): 1232-1233 |
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| Authors: | TANG Rui |
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| Affiliation: | TANG Rui(Department of Biology and Chemistry,Xingtai University,Xingtai 054001,Hebei,China) |
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| Abstract: | The optimum fermentation conditions of high-yield cellulolytic enzymes strain ZJW-6 were studied in this paper.The strain was cultured under different liquid fermentation conditions and enzymes activity of bacteria suspension was determined using DNS method.The results showed that the optimum fermentation conditions of ZJW-6 was as follows: peptone and(NH4)2SO4 as nitrogen source,shaking for 48h at 30℃ and pH 6. |
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| Keywords: | cellulose-decomposing microorganisms fermentation conditions cellulose enzyme activity |
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