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| 辣根叶片的离体培养 | |
| 引用本文： | 王晓峰,曹丽萍,宋卫丽,杨国放,张莹莹. 辣根叶片的离体培养[J]. 湖北农业科学, 2012, 51(11): 2361-2363 |
| 作者姓名： | 王晓峰 曹丽萍 宋卫丽 杨国放 张莹莹 |
| 作者单位： | 1. 沈阳农业科技开发院,沈阳,110866 2. 沈阳市东陵区农业中心,沈阳,110053 3. 辽宁省北票市东官营农业技术推广站,辽宁北票,122100 4. 沈阳农业大学园艺学院,沈阳,110866 |
| 基金项目： | 辽宁省教育厅创新团队项目 |
| 摘 要： | 以辣根(Armoracia rusticana (Lam.)Gaertn)叶片为外植体进行离体培养,考察了叶片采集时间、培养基中添加激素的种类和浓度对组织培养的影响.结果表明,2月份采集辣根叶片,组织培养污染率较小,为15％.愈伤组织诱导的适宜培养基为MS+0.5 mg/L 6-BA+0.2 mg/L NAA,出愈率为100.0％；叶片分化不定芽的适宜培养基为MS+0.5 mg/L 6-BA+1.0 mg/L NAA,分化率为80％；不定芽增殖的适宜培养基为MS+.0.2 mg/L KT;适宜的生根培养基为MS+0.2 mg/L NAA. |
| 关 键 词： | 辣根(A rmoracia rusticana(Lam.)Gaertn) 叶片 离体培养 |
In Vitro Culture of Armoracia rusticana Leaf |
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| WANG Xiao-feng , CAO Li-ping , SONG Wei-li , YANG Guo-fang , ZHANG Ying-ying. In Vitro Culture of Armoracia rusticana Leaf[J]. Hubei Agricultural Sciences, 2012, 51(11): 2361-2363 | |
| Authors: | WANG Xiao-feng CAO Li-ping SONG Wei-li YANG Guo-fang ZHANG Ying-ying |
| Affiliation: | 1.Shenyang Academy of Agricultural Technology Development,Shenyang 110866,China;2.Agricultural Center of Dongling District,Shenyang 110053,China;3.Agricultural Technology Extension Center of Dongguanying in Beipiao City,Beipiao 122100,Liaoning,China; 4.College of Horticulture,Shenyang Agricultural University,Shenyang 110866,China) |
| Abstract: | Tissue culture of Armoracia rusticana(Lam.)Gaertn was conducted using leaf as explant.Effects of sampling time,type and concentration of hormone on tissue culture were studied.Results showed that the pollution rate was the lowest(15%) when leaf sample was collected in Feburary.Optimum medium for different stage was callus induction medium,MS+0.5 mg/L 6-BA+0.2 mg/L NAA,with an induction ratio of 100.0%;differentiation medium,MS+0.5 mg/L 6-BA+1.0 mg/L NAA,with a differentiation rate of 80%;shoot enrichment medium,MS+0.2 mg/L KT;rooting medium,MS+0.2 mg/L NAA. |
| Keywords: | Armoracia rusticana(Lam.)Gaertn leaf in vitro culture |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |