| B型产气荚膜梭菌三种主要毒素基因的PCR检测 |
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| 引用本文： | 冶贵生. B型产气荚膜梭菌三种主要毒素基因的PCR检测[J]. 湖北农业科学, 2012, 51(13): 2873-2874 |
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| 作者姓名： | 冶贵生 |
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| 作者单位： | 青海大学农牧学院动物医学系,西宁,810016 |
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| 基金项目： | 青海大学高层次人才引进项目 |
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| 摘 要： | 为了对B型产气荚膜梭菌(Clostridium perfringens type B)进行快速检测,依据NCBI登录的产气荚膜梭菌α、B及ε毒素基因序列设计引物,并对其进行PCR扩增.琼脂糖凝胶电泳结果显示3个毒素基因均被成功扩增,目标片段条带清晰,长度与预期相符.
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| 关 键 词： | B型产气荚膜梭菌(Clostridium perfringens type B) α毒素 β毒素 ε毒素 PCR |
PCR Detection of Three Major Toxin Genes of Clostridium perfringens Type B |
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| YE Gui-sheng. PCR Detection of Three Major Toxin Genes of Clostridium perfringens Type B[J]. Hubei Agricultural Sciences, 2012, 51(13): 2873-2874 |
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| Authors: | YE Gui-sheng |
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| Affiliation: | YE Gui-sheng(Department of Veterinary Medicine,Agriculture and Animal Husbandry College,Qinghai University,Xining 810016,China) |
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| Abstract: | For the fast detection of Clostridium perfringens type B,primers of α,β and ε toxin genes were designed according to the reported sequences in NCBI;the genes were amplified by PCR.The results of agarose gel electrophoresis showed that the 3 genes were successfully amplified as the bands were clear and with right length. |
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| Keywords: | Clostridium perfringens type B α toxin β toxin ε toxin PCR |
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