耐高温酿酒酵母木薯乙醇发酵条件的优化

通过单因素试验和正交试验,对耐高温酿酒酵母(Saccharomyces cerevisiae)XG-1的木薯乙醇发酵的主要影响因素进行了研究.结果表明,在40℃高温培养条件下,XG-1对乙醇更加敏感,影响其木薯乙醇发酵,而影响木薯乙醇发酵各因素的主次顺序为发酵温度＞接种量=发酵时间.木薯乙醇发酵最佳工艺条件为发酵液中木薯淀粉含量200 g/L,氮素[(NH4)2SO4]添加量8 g/L,XG-1接种量约1.0×107CFU/mL,发酵温度33℃,发酵时间72h,发酵结束时发酵液中乙醇体积分教为13.1％,淀粉利用率为92.0％.

Optimization of the Cassava Alcohol Fermentation Conditions for Thermotolerant Saccharomyces cerevisiae

The impact factors of cassava alcohol fermentation by a thermotolerant strain XG-1 of Saccharomyces cerevisiae were studied via single factor experiments and orthogonal design.The results showed that in 40 ℃,XG-1 was more sensitive to alcohol that affecting the cassava fermentation,and the effect of each factor was fermentation temperature>inoculated cell number = fermentation time.The optimum alcohol fermentation conditions were XG-1 inoculated cell number 1.0×107 CFU/mL;cassava starch concentration,200 g/L;fermentation temperature,33 ℃;and fermentation time,72 h;additive amount of(NH4)2SO4,8 g/L.The volume fraction of ethanol reached 13.1%;and the starch utilization rate reached to 92.0% under these conditions.