| PCR仪法从林麝毛发中提取基因组DNA |
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| 引用本文： | 白康,任战军,王永奇. PCR仪法从林麝毛发中提取基因组DNA[J]. 湖北农业科学, 2012, 51(13): 2867-2870 |
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| 作者姓名： | 白康 任战军 王永奇 |
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| 作者单位： | 1. 西北农林科技大学动物科技学院,陕西杨凌,712100
2. 陕西省动物研究所,西安,710032 |
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| 基金项目： | 陕西省科学院基础研究项目 |
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| 摘 要： | 采用PCR仪法从林麝(Moschus berezovskii)毛发中提取基因组DNA,并与酚/氯仿法进行比较.结果表明,采用PCR仪法提取所得的DNA浓度高、纯度好.以提取所得的DNA为模板扩增林麝雄性激素受体基因外显子1,获得的PCR产物务带清晰、位置正确.PCR仅法与酚/氯仿法相比操作简单,提取所得的DNA质量较高,值得推广.
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| 关 键 词： | 林麝(Moschus berezovskii) 毛发 DNA提取 PCR仪法 |
Extracting DNA from Hair of Moschus berezovskii by PCR Cycler Method |
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| BAI Kang , REN Zhan-jun , WANG Yong-qi. Extracting DNA from Hair of Moschus berezovskii by PCR Cycler Method[J]. Hubei Agricultural Sciences, 2012, 51(13): 2867-2870 |
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| Authors: | BAI Kang REN Zhan-jun WANG Yong-qi |
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| Affiliation: | 1.College of Animal Science and Technology,Northwest A & F University,Yangling 712100,Shaanxi,China;2.Shaanxi Institute of Zoology,Xi’an 710032,China) |
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| Abstract: | PCR cycler method was adopt to extract genomic DNA from the hair of forest musk deer(Moschus berezovskii),and compared with phenol / chloroform method.The results showed that DNA extracted by PCR cycler method was with high concentration and purity.The exon 1 of androgen receptor in musk deer was amplified using the extracted DNA as a template;the PCR products showed clear bands at the correct position.Compared with phenol / chloroform method,PCR cycler method could be operated simply;and the DNA obtained was with high quality,thus worth to be popularized. |
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| Keywords: | Moschus berezovskii hair DNA extraction PCR cycler method |
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