| 大白菜开花基因 LFY的克隆及dsRNA抑制载体的构建 |
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| 引用本文： | 夏广清,何启伟,赵双宜,王翠花.大白菜开花基因 LFY的克隆及dsRNA抑制载体的构建[J].吉林农业大学学报,2004,26(6):615-619. |
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| 作者姓名： | 夏广清 何启伟 赵双宜 王翠花 |
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| 作者单位： | 山东农业大学园艺学院,泰安,270118;通化师范学院生物系,通化,134000;山东省农科院蔬菜所,济南,250100;山东大学生命科学院,济南,250200 |
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| 基金项目： | 山东省蔬菜良种产业化开发项目 |
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| 摘 要： | 以中国大白菜花蕾为材料，通过RT-PCR方法，参照GeneBank公布的序列克隆了Leafy(简称LFY)基因片段，测序分析结果表明，该基因与国外已经报道的序列具有94％的同源性。将此序列以相反方向插入到植物表达载体pROK2 35S启动子之间，并在2反向重复片段之间插入小麦乙酰辅酶A羧化酶的第20内舍子，从而构建了dsRNA抑制双元载体，该载体的构建为通过农杆菌介导转化大白菜进而解决早春扣反季栽培大白菜的先期抽薹问题创造了条件。
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| 关 键 词： | LFY基因 大白菜 dsRNA 抑制表达载体 |
| 文章编号： | 1000-5684(2004)06-0615-05 |
Cloning of Floral Gene LFY of Chinese Cabbage and Constructiong of dsRNA Inhibitor Vector |
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| XIA Guang-qing,HE Qi-wei,ZHAO Shuang-yi,WANG Cui-hua.Cloning of Floral Gene LFY of Chinese Cabbage and Constructiong of dsRNA Inhibitor Vector[J].Journal of Jilin Agricultural University,2004,26(6):615-619. |
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| Authors: | XIA Guang-qing HE Qi-wei ZHAO Shuang-yi WANG Cui-hua |
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| Abstract: | Through RT - PCR and according to the sequence on Gene Bank, the LFY gene was cloned from Chinese cabbage bud. Sequence analysis showed that it was of 94% homology with the gene reported abroad. When the inverse gene fragment was inserted into 35S promoter of pROK2 plant expression vector and into the 20th intron of AS - COA carboxylase of wheat, dsRNA inhibitor vector was constructed. |
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| Keywords: | LFY gene Chinese cabbage dsRNA inhibitor expression vector |
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