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| 家蚕磷酸甘油醛异构酶Tpi基因的克隆 | |
| 引用本文： | 牛宝龙,孟智启,翁宏飙,沈卫锋,吕顺霖. 家蚕磷酸甘油醛异构酶Tpi基因的克隆[J]. 蚕业科学, 2005, 31(1): 47-52 |
| 作者姓名： | 牛宝龙 孟智启 翁宏飙 沈卫锋 吕顺霖 |
| 作者单位： | 1. 浙江大学动物科学学院,杭州,310029;浙江省农业科学院蚕桑研究所,杭州,310021 2. 浙江省农业科学院蚕桑研究所,杭州,310021 3. 浙江大学动物科学学院,杭州,310029 |
| 摘 要： | 利用生物信息学方法成功地克隆了家蚕磷酸甘油醛异构酶(triosephosphateisomeraseTpi)基因(GenBank登记号为AY734490)。该基因全长为2875bp;含有5个外显子、4个内含子,所有内含子/外显子边界都符合典型的GT/AG剪切模式;cDNA序列全长1051bp,并经RTPCR克隆和序列分析进行了验证;具有完整的开放阅读框架(ORF,95~839bp),可编码248个氨基酸的蛋白。通过与NCBI蛋白数据库的比对表明,该基因编码的蛋白为磷酸甘油醛异构酶(EC5311),参与葡萄糖分解代谢,即将磷酸二羟丙酮转化为3磷酸甘油醛,在果蝇、蚊子、黄粉虫、美洲淤夜蛾、棉铃虫等昆虫中具有高度的保守性。 |
| 关 键 词： | EST数据库 PCR 磷酸甘油醛异构酶 家蚕 |
| 文章编号： | 0257-4799(2005)01-0047-06 |
| 修稿时间： | 2004-09-07 |
Cloning of Triosephoshpate Isomerase Gene of the Silkworm,Bombyx mori |
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| NIU Baolong,MENG Zhiqi,WENG Hongbiao,SHEN Weifeng,U Shunlin. Cloning of Triosephoshpate Isomerase Gene of the Silkworm,Bombyx mori[J]. Acta Sericologica Sinica, 2005, 31(1): 47-52 | |
| Authors: | NIU Baolong MENG Zhiqi WENG Hongbiao SHEN Weifeng U Shunlin |
| Abstract: | A novel silkworm,Bombyx mori,triosephosphate isomerase(Tpi) gene is cloned by blasting its EST database and PCR test,and its sequence has been deposited in the GenBank (Accession No: AY734490).It has five extrons and four introns spanning 2 875 bp in full length and all the boundary of extrons/introns has GT/AG conserved sequences.Its 1 051bp mRNA has a complete open reading fragment (ORF: 95-834) and encodes 248 Aa protein,which is triosephosphate isomerase(EC 5.3.1.1)for catalysis of conversion dihydroxyacetone phosphate into glyceraldehydes 3-phosphate in glucose glycolysis and very conserved in many insects such as Heliothis virescens,Helicoverpa armigera,Tenebrio molito,Anopheles gambiae and Drsophila melanogaster. |
| Keywords: | EST database PCR Triosephosphate isomerase Bombyx mori |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |