| 建兰种质资源遗传多样性分析及核心种质构建 |
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| 引用本文： | 陈明堃,陈璐,孙维红,马山虎,兰思仁,彭东辉,刘仲健,艾叶.建兰种质资源遗传多样性分析及核心种质构建[J].园艺学报,2022(1):175-186. |
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| 作者姓名： | 陈明堃 陈璐 孙维红 马山虎 兰思仁 彭东辉 刘仲健 艾叶 |
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| 作者单位： | 福建农林大学园林学院;福建农林大学林学院 |
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| 基金项目： | 国家重点研发计划项目(2019YFD1000400);福建农林大学杰出青年科研人才计划项目(xjq201910);国家自然科学基金项目(31700618)。 |
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| 摘 要： | 研究记录了311份建兰(Cymbidium ensifolium)种质资源的萼片、花瓣、唇瓣颜色以及叶艺、瓣型和唇瓣斑点等质量性状的表型特征,并对其进行赋值和统计分析;利用22对SSR荧光引物对311份建兰种质资源进行PCR扩增,分析其遗传多样性;利用Structure软件分析建兰种质资源的群体结构,采用主成分分析和聚...
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| 关 键 词： | 建兰 种质资源 表型性状 SSR 遗传多样性 核心种质 |
Genetic Diversity Analysis and Core Collection of Cymbidium ensifolium Germplasm Resources |
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| CHEN Mingkun,CHEN Lu,SUN Weihong,MA Shanhu,LAN Siren,PENG Donghui,LIU Zhongjian,AI Ye.Genetic Diversity Analysis and Core Collection of Cymbidium ensifolium Germplasm Resources[J].Acta Horticulturae Sinica,2022(1):175-186. |
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| Authors: | CHEN Mingkun CHEN Lu SUN Weihong MA Shanhu LAN Siren PENG Donghui LIU Zhongjian AI Ye |
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| Institution: | (Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization,College of Landscape Architecture,Fujian Agriculture and Forestry University,Fuzhou 350002,China;College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,China) |
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| Abstract: | The phenotypic characteristics of sepal color,petal color,lip color,leaf art,petal type and lip spot of 311 Cymbidium ensifolium germplasms were recorded,and the traits were assigned and statistically analyzed.Twenty-two pairs of SSR fluorescent primers were used to perform PCRamplification on 311 C.ensifolium germplasm resources in order to conduct genetic diversity analysis.Use Structure software to analyze the population structure of C.ensifolium germplasm resources,and principal component analysis and cluster analysis were employed for verification.Core collection was analyzed and screened by using Core Finder software and t-test.The results showed that the phenotypic genetic diversity of the six quality traits of 311 C.ensifolium germplasm resources were all greater than 0.5,indicating that the phenotype of C.ensifolium germplasm resources had abundant genetic variations.A total of 171 alleles(Na)were detected by 22 pairs of SSR fluorescent primers,the average of Ncwas 2.729.High genetic diversity was revealed in the germplasm resources of C.ensifolium(I=1.026).Structure software analysis results showed that the optimal population number K was three,and there is a small amount of germplasm mixing among the populations.The population structure analysis divided C.ensifolium germplasm resources into three groups.Principal component analysis and cluster analysis further verified the reliability of its classification.In this study,51 core collections of C.ensifolium were constructed,accounting for 16.4%of the initial collection,with the retention rate of Na,Nc,and I were 100%,130%and 124%,respectively.Through t-test and principal coordinate analysis showed that there was no significant difference between the genetic diversity of the core collection and initial collection.The 51 core collections constructed could represent the genetic diversity of C.ensifolium germplasm resources to the greatest extent. |
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| Keywords: | Cymbidium ensifolium germplasm resources phenotypic traits SSR genetic diversity core collection |
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