三种镰孢菌对不同苜蓿品种致病性的比较研究

为了进一步了解根腐病病原菌的生物学特性以及不同病原菌对苜蓿的致病性,本研究以苜蓿根腐病病原BF1(Fusarium equiseti)、BF28(F.oxysporum)、BF36-2(F.solani)三种镰孢菌菌株为试验材料,观测菌落的外观特征与产孢量,并对WL343、金皇后、阿尔冈金、WL525四个苜蓿品种以菌液...

Comparative Study on Pathogenicity of Three Fusarium Strains to Different Alfalfa Varieties

In order to further investigate the biological characteristics of root rot pathogens and the pathogenicity of different pathogens to alfalfa,three root rot pathogens including BF1(Fusarium equiseti),BF28(F.oxysporum) and BF36-2(F.solani) were selected as experimental materials to investigate their bio-characteristics and reproductive capacities.Four alfalfa varieties of WL343,Golden Empress,Algonguin and WL525 were inoculated by fungal suspension and fungal cakes.The results showed that alfalfa was effected more rapidly and more severely by using fungal suspension in compared with using fungal cakes.Different strains had different colonization abilities in alfalfa root system.The mycelium of BF1 gathered near the roots,but that of BF28 and BF36-2 did not gather.In the form of bacterial cake inoculation,the pathogenicity of three strains was different.The strains with strong sporulation ability had strong pathogenicity.The pathogenicity of BF28 was the strongest,followed by BF36-2,and BF1 was the weakest.Infection process and interaction access between host plant and Fusarium were studied through anatomical structure.Pathogens invaded into epidermal cell with chlamydospores.They spread in the intercellular in the form of myclium or spores.Spores released toxin that dissolving the cell wall and decaying the host tissue in finally,root pulp became hollow and the leaves appeared ulcerative spots,plant growth was hindered.