紫花苜蓿根际土壤细菌群落对腐熟牛粪响应

为了探讨腐熟牛粪施用量对作物根际土壤细菌丰度、多样性及群落结构的影响,本研究以盆栽紫花苜蓿(Medicago sativa L.)为试验材料,分别添加0％,3％,6％,9％的腐熟牛粪,在添加30 d,60 d,90 d后进行取样,利用Illumina-Miseq高通量测序平台对土壤细菌16S rRNA V3-V4区进行...

Bacteria Communities of Medicago sativa Rhizosphere Soil in Response to Composted Cow Manure

In order to determine the effects of the application amount of composted cow manure on the community diversity and structure of crop rhizosphere soil bacteria,this study sampled potted alfalfa (Medicago sativa L.) soils supplemented with 0%,3%,6%,and 9% composted cow manure for 30 d,60 d,and 90 d respectively,sequenced V3-V4 region of soil bacterial 16S rRNA using Illumina-Miseq high-throughput. The results showed that Actinobacteria and Proteobacteria were the dominant phyla in all tested samples. Addition of composted cow manure decreased the diversity and richness of soil bacteria (P<0.05),significantly increased the abundance of Firmicutes,Bacteroidetes,and Proteobacteria,as well as increased the abundance of Bacillus and Actinomadura (P<0.05). Cluster analysis showed that the abundance of bacterial genus abundance was similar between the whole soil and the whole manure samples. The addition of composted cow manure significantly affected the abundance of some dominant bacteria. The abundance of genera Lysobacter,Bacillus,Saccharomonospora,Planifilum,Cellvibrio,Steroidobacter,Devosia in the mixed samples were significantly higher than those in the non-fertilization group,the whole soil as well as the whole manure (P<0.05). The number of soil bacterial OTU decreased with supplemental proportions,but the number of soil bacterial OTU increased with the growth of alfalfa at 6% cow manure application rate and decreased continuously at 9% manure application rate.