噬菌体裂解酶对白羽肉鸡生长性能、器官指数及血清抗氧化酶活性的影响

为评估噬菌体裂解酶作为一种替抗饲料添加剂应用于畜禽生产的安全性及其应用潜能,将3日龄健康白羽肉鸡在饲喂基础饲粮上添加高剂量(200 mg/kg)、中剂量(100 mg/kg)和低剂量(50 mg/kg)的噬菌体裂解酶制剂(由TSPphg和MMPphg噬菌体裂解酶制得),并将抗生素产品金霉素制剂以50 μg/g的剂量添加...

Effects of phagelyase on growth performance,organ index and major antioxidant enzyme activities in white-feather broilers

This study was to evaluate the safety and application potential of bacteriophage lyase as an alternative feed additive for livestock and poultry. Three-day-old healthy white-feather broilers were fed with high(200 mg/kg), medium(100 mg/kg), and low(50 mg/kg) doses of phage lyase preparations(from TSPphg And MMPphg bacteriophage lyases), and the antibiotic product chlortetracycline was added to the diet at a dose of 50 μg/g as a positive control, and the basal diet was used as a blank control. Then, the changes in the feed-to-weight ratios of white feather broilers were recorded within 28 d from the beginning of the experiment, and the indices of their immune organs(spleen, and bursa) and digestive organs(duodenum, jejunum, ileum) were collected from each group of the tested broilers at 28 d of the experimental feeding, and the main antioxidant enzyme indices(SOD, GSH-Px, and CAT) in their serum were measured. The results showed that with the increase in the dosage of compound phage lyase,the feed/weight ratio,and the jejunum and ileum indexes of the tested white feather broilers gradually decreased,and the main antioxidant enzyme activities( GSH-Px,CAT,and SOD) in their serum gradually increased,as compared with the blank group( P<0. 05). The activities of catalase( CAT) and superoxide dismutase( SOD) in the serum of the treated white feathered broilers were significantly better than those of the chlortetracycline group( P<0. 05). The above results indicated that the phage cleavage enzyme was quite safe and could better promote the intestinal absorption and immunity of livestock and poultry,compared with the antibiotic products,and the enzyme could be used as a favorable alternative feed additive in broiler breeding.