| 适用于rDNA ITS分析的兰属菌根真菌培养及DNA提取方法 |
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| 引用本文： | 庄彩云,李潞滨,胡陶,唐征,刘振静,杨凯. 适用于rDNA ITS分析的兰属菌根真菌培养及DNA提取方法[J]. 北京农学院学报, 2007, 22(3): 4-6,31 |
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| 作者姓名： | 庄彩云 李潞滨 胡陶 唐征 刘振静 杨凯 |
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| 作者单位： | 中国林业科学研究院,林业研究所,国家林业局林木培育实验室,北京,100091;北京农学院,植物科学技术系,北京,102206;北京农学院,生物技术系,北京,102206;农业应用新技术北京市重点实验室,北京,102206 |
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| 基金项目： | 引进国际先进农业科技计划(948计划) , 北京市属市管高校人才强教计划 |
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| 摘 要： | 为探讨兰属植物与其共生真菌之间专一性关系,采用覆盖纤维素滤膜于PDA培养基的方法培养兰属菌根真菌,提取其DNA后用于rDNA ITS分析。在培养过程中克服PDA液体培养及固体培养获得菌丝体少的缺点,操作方便,获得大量菌丝体;采用改进的提取植物基因组DNA的SDS方法提取真菌的DNA,通过提高缓冲液的pH值、增加EDTA的浓度以提高DNA的提取效果。结果表明,此培养方法及改进的DNA提取方法均简便易行,提取的DNA产量高、质量较好;可满足菌根真菌rDNA ITS分析的要求。
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| 关 键 词： | 兰属菌根真菌 醋酸纤维素滤膜 真菌DNA |
| 文章编号： | 1002-3186（2007）03-0004-03 |
| 修稿时间： | 2007-03-21 |
A Rapid and Simple Method for Culturing of Cymbidium mycorrhizal Fungi and Extracting of DNA from the Fungi for the Analysis of rDNA ITS |
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| ZHUNAG Cai-yun,LI Lu-bin,HU Tao,TANG Zheng,LIU Zhen-jing,YANG Kai. A Rapid and Simple Method for Culturing of Cymbidium mycorrhizal Fungi and Extracting of DNA from the Fungi for the Analysis of rDNA ITS[J]. Journal of Beijing Agricultural College, 2007, 22(3): 4-6,31 |
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| Authors: | ZHUNAG Cai-yun LI Lu-bin HU Tao TANG Zheng LIU Zhen-jing YANG Kai |
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| Affiliation: | 1. Key Laboratory of Tree Breeding and Eultivation,State Forestry Administration,Institute of Forestry, Chinese Academy of Forestry, Beijing 100091 ,China; 2. a. Department of Plant Science and Technology; b. Department of Biological Technology, Beijing University of Agriculture,Beijing 102206, China; 3. New Technological Laboratory in Agriculture Application in Beijing,102206, China |
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| Abstract: | In order to analyze the specificity relationship between the Cymbidium and the Cymbidium mycorrhizal fungi,a rapid method for culturing the Cymbidium mycorrhizal fungi on PDA covered with cellulose acetate membrane was reported in this paper.The extracted DNA of Cymbidium mycorrhizal fungi was used for the rDNA ITS analysis.The method obtained a great quantity hyphostroma.The improved SDS method which used in plant genomic DNA was applied in extraction of fungi DNA.The DNA was satisfied for the rDNA ITS analysis of the Cymbidium mycorrhizal fungi. |
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| Keywords: | Cymbidium mycorrhizal fungi cellulose acetate membrane fungi DNA extraction |
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