Effects of donor plant temperature,photoperiod, and age on anther culture response of Capsicum annuum L. |
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Authors: | Kell Kristiansen Sven Bode Andersen |
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Institution: | (1) Department of Plant Breeding and Propagation, Research Centre for Horticulture, Kirstinebjergvej 10, DK-5792 Årslev, Denmark;(2) Section of Plant Breeding and Biotechnology, The Royal Veterinary & Agricultural University, Thorvaldsensvej, DK-1871 Frederiksberg C, Denmark |
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Abstract: | Summary Effects of temperature and photoperiod for donor plant growth on embryo formation in Capsicum annuum anther culture were investigated. Donor plants were grown in glasshouses at minimum temperatures between 16 and 30° C and at photoperiods between 11 and 19 h. Anthers were collected from individual plants over five to nine week periods to test the significance of donor plant age. Embryos were obtained at all temperature regimes with a calculated optimum temperature of 26.4° C. Embryo formation was unaffected by the photoperiods tested. Embryo formation varied among successive samplings. However, a significant decline in anther culture response with increasing donor plant age was observed in all three experiments.Abbreviations AT
average air temperature the week before anther incubation
- EMB 100
1n (embryos per 100 anthers +1) |
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Keywords: | Anther culture Capsicum annuum donor plant pepper plant age temperature |
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