| 犬瘟热病毒在MA-104、Marc-145、Vero3种细胞内增殖效果的比较 |
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| 引用本文： | 王朝蕾,崔卓宇,吕永钢,胡江锋,焦铁军,樊军喜,王根红,崔瑞宁. 犬瘟热病毒在MA-104、Marc-145、Vero3种细胞内增殖效果的比较[J]. 畜牧与饲料科学, 2012, 33(10): 7-8 |
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| 作者姓名： | 王朝蕾 崔卓宇 吕永钢 胡江锋 焦铁军 樊军喜 王根红 崔瑞宁 |
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| 作者单位： | 杨凌绿方生物工程有限公司,陕西杨凌,712100 |
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| 摘 要： | 试验比较了MA-104、Marc-145、Vero 3种猴肾细胞增殖犬瘟热病毒的敏感性,按常规方法将犬瘟热病毒(CDV-XN112株)分别接种于MA-104、Marc-145、Vero 3种细胞,通过观察细胞病变,确定收毒时间,比较病毒滴度。结果表明,犬瘟热病毒可在MA-104、Marc-145、Vero 3种细胞内增殖,并出现典型细胞病变,效价均达到104.5TCID50/0.1mL以上,而Vero细胞增殖犬瘟热病毒更为经济高效,是增殖该病毒的理想细胞。
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| 关 键 词： | 犬瘟热病毒 细胞培养 病毒含量 |
Comparison on Multiplication Effect of Canine Distemper Virus in MA-104, Marc-145, and Vero Cells |
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| WANG Zhao-lei,CUI Zhuo-yu,Lu Yong-gang,HU Jiang-feng,JIAO Tie-jun,FAN Jun-xi,WANG Gen-hong,CUI Rui-ning. Comparison on Multiplication Effect of Canine Distemper Virus in MA-104, Marc-145, and Vero Cells[J]. Animal Husbandry and Feed Science, 2012, 33(10): 7-8 |
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| Authors: | WANG Zhao-lei CUI Zhuo-yu Lu Yong-gang HU Jiang-feng JIAO Tie-jun FAN Jun-xi WANG Gen-hong CUI Rui-ning |
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| Affiliation: | (Yangling Lvfang Bio-engineering Co., Ltd.,Yangling 712100,China) |
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| Abstract: | After canine distemper virus （CDV-XN 112 strain） was inoculated into MA- 104, Marc- 145 and Vero cells, the sensitivity of three types of monkey kidney to CDV was compared by observing cell pathological effect, determining the time for harvesting virus and comparing virus titers. The results showed that three cells were suitable for CDV, with typical cell pathological effect, and the titers of virus harvest fluids were more than 104.5 TCID50/0. lmL, among them Vero cell was more cost-effective and ideal for proliferation CDV. |
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| Keywords: | canine distemper virus cell culture virus content |
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