| H9N2亚型禽流感流行株灭活疫苗种毒的筛选 |
| |
| 引用本文： | 万晓朋,曾显营,田国彬,李雁冰,邓国华,施建忠,刘丽玲,钟功勋,姜永萍,王彦妮,陈化兰. H9N2亚型禽流感流行株灭活疫苗种毒的筛选[J]. 中国预防兽医学报, 2010, 32(4) |
| |
| 作者姓名： | 万晓朋 曾显营 田国彬 李雁冰 邓国华 施建忠 刘丽玲 钟功勋 姜永萍 王彦妮 陈化兰 |
| |
| 作者单位： | 中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室/农业部动物流感重点开放实验室,黑龙江,哈尔滨,150001 |
| |
| 基金项目： | 科技基础性工作专项项目，国家科技支撑计划，哈尔滨市科技攻关项目，现代农业产业技术体系建设 |
| |
| 摘 要： | 为筛选出具有良好免疫原性的禽流感病毒(AIV)H9N2亚型灭活流行株种毒,选择2008年中国大陆8个省份15株H9N2亚型AIV分离株进行抗原性分析,选取代表流行株进行鉴定并制备灭活疫苗,进行免疫效力评估。实验结果显示,2008年分离株之间抗原性比较接近,与2000年前分离株的抗原性相差较大;2008年分离的8株病毒HA基因核苷酸同源率在93.2%～98.6%之间,而CK/SH/10/01毒株与这8株病毒的同源率仅在91.9%～93.5%之间;CK/ZJ/17/08、CK/SD/2CZ/08、DK/FJ/560/08、CK/FJ/521/08、CK/HuN/174/08和CK/HuN/33/08候选株病毒在SPF鸡胚上连续传15代HA价及致病性等均未改变,SPF鸡鼻腔感染106EID50各毒株后均无任何症状和死亡出现;候选株灭活疫苗免疫SPF鸡3周时,产生针对疫苗株抗原检测的HI抗体介于10.35log2～11.811log2,以106EID50剂量鼻腔感染途径攻毒后,只有CK/HuN/174/08和CK/HuN/33/08株灭活疫苗免疫鸡不仅可以对同源毒株的攻击提供良好的免疫保护,而且对2008年分离的异源毒株的攻击也能提供比较理想的免疫保护,可作为适合我国大部分地区应用的H9N2亚型禽流感灭活疫苗种毒株。
|
| 关 键 词： | 禽流感 H9N2亚型 灭活疫苗 种毒 筛选 |
Selection and evaluation of H9N2 avian influenza inactivate vaccine candidates |
| |
| WAN Xiao-peng,ZENG Xian-ying,TIAN Guo-bin,LI Yan-bing,DENG Guo-hua,SHI Jian-zhong,LIU Li-ling,ZHONG Gong-xun,JIANG Yong-ping,WANG Yan-ni,CHEN Hua-lan. Selection and evaluation of H9N2 avian influenza inactivate vaccine candidates[J]. Chinese Journal of Preventive Veterinary Medicine, 2010, 32(4) |
| |
| Authors: | WAN Xiao-peng ZENG Xian-ying TIAN Guo-bin LI Yan-bing DENG Guo-hua SHI Jian-zhong LIU Li-ling ZHONG Gong-xun JIANG Yong-ping WANG Yan-ni CHEN Hua-lan |
| |
| Affiliation: | WAN Xiao-peng,ZENG Xian-ying,TIAN Guo-bin,LI Yan-bing,DENG Guo-hua,SHI Jian-zhong,LIU Li-ling,ZHONG Gong-xun,JIANG Yong-ping,WANG Yan-ni,CHEN Hua-lan(Animal Influenza Laboratory of the Ministry of Agriculture/State Key Laboratory of Veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China) |
| |
| Abstract: | In this study,candidate vaccine strains were selected from fifteen H9N2 subtype avian influenza viruses(AIV) isolated from eight provinces in mainland China during 2008. Six vaccine strains including CK/ZJ/17/08,CK/SD/2CZ/08,DK/FJ/560/08,CK/FJ/521/08,CK/HuN/174/08 and CK/HuN/33/08 were selected based on the antigenic analysis. The immune efficacy of the strains was evaluated by inoculaing SPF chickens with oil-emulsion inactivated vaccines prepared from respective strains. The results showed that all vaccin... |
| |
| Keywords: | Avian influenza H9N2 subtype inactivated vaccine vaccine candidate selection |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
