Benomyl sensitivity assays and species-specific PCR reactions highlight association of two <Emphasis Type="Italic">Colletotrichum gloeosporioides</Emphasis> types and <Emphasis Type="Italic">C. acutatum</Emphasis> with rumple disease on Primofiori lemons |
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Authors: | Manuel Valero Santiago García-Martínez Manuel J Giner Aranzazu Alonso Juan J Ruiz |
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Institution: | 1.Departamento de Producción Vegetal y Microbiología,Universidad Miguel Hernández (UMH) - Campus de Orihuela. Escuela Politécnica Superior de Orihuela (EPSO),Alicante,Spain;2.Departamento de Biología Aplicada,Universidad Miguel Hernández (UMH) - Campus de Orihuela. Escuela Politécnica Superior de Orihuela (EPSO),Alicante,Spain |
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Abstract: | Rumple is a serious peel collapse of Primofiori lemons in the southeast of Spain with an unresolved aetiology. Symptoms typically
occur on fruits at ripening under wet conditions as dark sunken lesions producing premature fruit drop and damaged fruits
unacceptable for fresh commercialization. A total of 16 Colletotrichum spp. isolates established from rumple-affected lemons collected during the autumn of 2007 from two different orchards were
characterized by molecular and phenotypic assays and compared with reference isolates. Species-specific PCR reactions using
β-tubulin 2 nucleotide sequences showed Colletotrichum gloeosporioides to predominate (81.5%) with limited occurrence of C. acutatum (18.75%). Among the C. gloeosporioides isolates, five (38.5%) showed benomyl resistance and eight (61.5%) were highly sensitive to the fungicide. The limited occurrence
of C. acutatum could be related to factors such as the presence of both species on the same fruit, unfavourable meteorological conditions
and low disease incidence. This work reveals an association of C. gloeosporioides and C. acutatum isolates with rumple disease of lemons and expands the range of C. acutatum on citrus. |
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