Molecular characterization of the phytoplasmas associated with toon trees and periwinkle in India |
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| Authors: | Omar F. Ayman Yogesh Kumar Vipin Hallan Aijaz A. Zaidi |
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| Affiliation: | (1) Department of Plant Pathology, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt;(2) Plant Virus Laboratory, Floriculture Division, Institute of Himalayan Bioresource Technology, Council of Scientific and Industrial Research, Palampur, 176061, Himachal Pradesh, India; |
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| Abstract: | In November 2008 in Himachal Pradesh and Chandigarh regions in India, toon trees and periwinkles were observed to have formed short internodes, small leaves and witches’-broom symptoms, typical of phytoplasma infection. The symptomatic toon and periwinkle samples were tested with universal PCR tests, and the 16S rRNA, rplB-rpsC, secA and secY genes were sequenced. The causal agents belonged to subgroup 16SrI-B of ‘Candidatus Phytoplasma asteris’, based on 16S rDNA, ribosomal protein gene, secA and secY phylogenetic analysis. |
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