小麦TaPYL8基因的克隆与抗旱功能分析

脱落酸(ABA)信号通路在植物对干旱胁迫的响应中扮演着至关重要的角色；作为ABA受体，PYL家族蛋白在ABA信号转导中发挥核心作用。小麦TaPYL8是PYL家族的成员。为了明确TaPYL8是否参与小麦干旱胁迫响应，本研究分析了TaPYL8的表达模式，创建过表达TaPYL8拟南芥，并探讨了干旱对拟南芥生理生化及胁迫相关基因表达的影响。结果表明，TaPYL8表达量在干旱胁迫、外源ABA及PEG6000处理后上调。干旱胁迫下，过表达TaPYL8增强了拟南芥的存活率，降低了叶片失水率和MDA含量，提高了SOD、POD和CAT等抗氧化酶活性及AtSOD、AtP5CS1、AtABF3等胁迫相关基因的表达量。推测TaPYL8通过促进胁迫相关基因表达增强植物抗旱能力。

Cloning and Drought Resistance Function Analysis of Wheat TaPYL8 Gene

Abscisic acid(ABA) signaling pathway plays an important role in plant responding to drought stress. As the receptor of ABA, the PYL protein family is the core component of the signal pathway and plays a key role in ABA signal transduction. In order to clarify the potential functions of TaPYL8 in plant responding to drought stress, the expression pattern of TaPYL8 was analyzed via qRT-PCR, and TaPYL8 were overexpressed in Arabidopsis to explore the impact of drought stress on wheat physiology and the expression of drought stress related genes. The results showed that the expression levels of TaPYL8 were increased under drought stress, exogenous ABA and PEG6000 treatments. Importantly, overexpression of TaPYL8 led to an enhanced drought stress tolerance because the Arabidopsis overexpression line had higher survival rate after drought stress treatment and lower water loss rate of leaves, MDA content and superior SOD, POD and CAT activities under drought stress, which might be related to the higher expression levels of stress related genes, such as AtSOD, AtP5CS1, AtABF3, and etc. Our results indicated that TaPYL8 conferred drought stress tolerance in Arabidopsis via promoting stress related genes expression.