| 聚乙二醇8000对棉铃虫微粒体蛋白沉淀作用的研究 |
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| 引用本文： | 郑明奇,张文吉,邱星辉,冷欣夫.聚乙二醇8000对棉铃虫微粒体蛋白沉淀作用的研究[J].农药学学报,2005,7(1):81-84. |
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| 作者姓名： | 郑明奇 张文吉 邱星辉 冷欣夫 |
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| 作者单位： | 中国科学院,动物研究所,农业虫害鼠害综合治理研究国家重点实验室,北京100080;中国农业大学,应用化学系,农业部农药化学及使用技术重点实验室,北京100094;中国农业大学,应用化学系,农业部农药化学及使用技术重点实验室,北京100094;中国科学院,动物研究所,农业虫害鼠害综合治理研究国家重点实验室,北京100080 |
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| 基金项目： | 国家自然科学基金资助项目(30270885). |
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| 摘 要： | 为建立棉铃虫Helicoverpa armigera微粒体P450的分离纯化方案,比较了不同浓度下聚乙二醇8000(PEG8000)对棉铃虫幼虫中肠和脂肪体微粒体蛋白的沉淀作用。结果表明,终浓度为8.0×104 mg/L的PEG8000可以使中肠和脂肪体微粒体蛋白的78%沉淀下来,其中包含的细胞色素P450分别占中肠和脂肪体P450总量的28%和34%。SDS-PAGE显示,中肠微粒体经8.0×104 mg/L的PEG8000沉淀后,被沉淀蛋白的分子质量主要集中在14.1 ~ 40 kD和66 ~97 kD范围内。
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| 关 键 词： | 棉铃虫 细胞色素P450 聚乙二醇沉淀 |
| 文章编号： | 1008-7303(2005)01-0081-04 |
| 收稿时间： | 2004/7/23 0:00:00 |
| 修稿时间： | 2004年7月23日 |
Precipitation of Micorsomal Proteins in Helicoverpa armigera by PEG8000 |
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| ZHENG Ming-qi,ZHANG Wen-ji,QIU Xing-hui and LENG Xin-fu.Precipitation of Micorsomal Proteins in Helicoverpa armigera by PEG8000 [J].Chinese Journal of Pesticide Science,2005,7(1):81-84. |
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| Authors: | ZHENG Ming-qi ZHANG Wen-ji QIU Xing-hui and LENG Xin-fu |
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| Institution: | 1.State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology, the Chinese Academy of Sciences, Beijing 100080, China2.Key Laboratory of Pesticide Chemistry and Application Technology, Department of Applied Chemistry, China Agricultural University, Beijing 100094, China |
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| Abstract: | In order to develop a protocol for purifying cytochrome P450 in the cotton bollworm, Helicoverpa armigera, the precipitation results of PEG8000 at different concentration to microsomal proteins of cotton bollworm were compared. The results showed that at the concentration of 8.0×104 mg/L, 78% microsomal proteins including 28% total P450 of midgut microsome or 34% total P450 of fatbody microsome were precipitated. SDS-PAGE showed that most of the precipitated microsomal proteins from midgut were those which molecular weight was among the range of 14.1 ~40 kD and 66 ~97 kD. |
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| Keywords: | Helicoverpa armigera cytochrome P450 PEG precipitation |
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