马铃薯试管块茎诱导过程中总RNA的变化

试验以南中 5 5 2 (N5 5 2 )脱毒试管苗为材料 ,设计采用了 5种蔗糖浓度和 5种光照时间共 2 5个处理 ,测定分析了各处理试管块茎诱导前后总RNA的变化规律。结果显示 ,除连续光照下各蔗糖浓度处理的总RNA含量变化幅度较小外 ,其它处理在培养过程中总RNA含量均存在不同程度的变化差异。光照处理后总RNA含量上升的处理能正常形成块茎。培养期间总RNA含量上升到一定程度后 ,即呈下降趋势的处理无块茎形成或形成极少。不同处理可能启动了不同的基因表达系统 ,进而导致了试管块茎的形成差异

RNA VARIATION DURING MICRORUBER INDUCTION OF POTATOES

Virus free plantlets of cv. N552 of potato(S.tuberosum) produced in vitro were used for the experiments. The variation of the total RNA was analysed before and after microtuberization for twenty-five treatments with different sucrose concentrations and day-lenghts. The results showed that a marked variation in RNA concentration was observed in all the treatments except for the plantlets growing at consecutive light. Microtubers formed in the treatments that showed increase in RNA concentration after the induction, and no or less tubers accompanied with the treatments in which RNA concentration increase initially and then sharply declined. It was assumed that difference in tuber formation may result from a systematic difference in gene expression that was caused by the induction treatments.