| 鸡J亚型白血病病毒荧光定量PT-PCR检测方法的建立 |
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| 引用本文： | 林甦,朱春华,陈珍,刘斌琼,施少华,江斌,蔡国漳,林羽,黄瑜.鸡J亚型白血病病毒荧光定量PT-PCR检测方法的建立[J].福建农业学报,2010,25(3):289-293. |
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| 作者姓名： | 林甦 朱春华 陈珍 刘斌琼 施少华 江斌 蔡国漳 林羽 黄瑜 |
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| 作者单位： | 福建省农业科学院畜牧兽医研究所,福建,福州,350013 |
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| 摘 要： | 运用实时荧光定量PCR技术,针对鸡J亚型白血病的gp85基因设计特异性引物,建立了一种快速准确诊断鸡J亚型白血病病毒(ALV-J)的荧光定量PT-PCR方法。通过重复性、特异性和灵敏度等验证表明,该方法适用于ALV-J的临床检测,为ALV-J引起的鸡肿瘤性疾病的快速诊断及采取相应的治疗措施提供了重要的技术支撑。
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| 关 键 词： | 鸡白血病 荧光定量PT-PCR gp85基因 |
Quantitative detection of ALV-J by fluorescence RT-PCR |
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| LIN Su,ZHU Chun-hua,CHEN Zhen,LIU Bin-qiong,SHI Shao-hua,JIANG Bin,CAI Guo-zhang,LIN Yu,HUANG Yu.Quantitative detection of ALV-J by fluorescence RT-PCR[J].Fujian Journal of Agricultural Sciences,2010,25(3):289-293. |
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| Authors: | LIN Su ZHU Chun-hua CHEN Zhen LIU Bin-qiong SHI Shao-hua JIANG Bin CAI Guo-zhang LIN Yu HUANG Yu |
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| Institution: | LIN Su,ZHU Chun-hua,CHEN Zhen,LIU Bin-qiong,SHI Shao-hua,JIANG Bin,CAI Guo-zhang,LIN Yu,HUANG Yu(Institute of Animal Husbandry & Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China) |
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| Abstract: | Avian leukosis is one of the main chicken tumor diseases.It causes considerable economic loss for poultry farmers.A new,rapid diagnostic mehtod applying the real-time florescence quantitative polymerase chain reaction(PT-PCR) was developed for detecting the avian leukosis virus subgroup J(ALV-J).A set of subgroup J special primers for gp85 gene was used.Validations on repeatability,specificity and sensitivity of the methodology showed its potential applicability for clinical diagnosis of ALV-J. |
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| Keywords: | avian leukosis RT-PCR gp85 gene |
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