|
|||||
|
|
| 番茄温敏型核雄性不育系T-4 花药蛋白质 | |
| 引用本文： | 国亚慧,于分弟,甘桂云,韦冠睦,王先裕,杜永臣,桝田正治. 番茄温敏型核雄性不育系T-4 花药蛋白质[J]. 中国蔬菜, 2013, 1(6): 58-62 |
| 作者姓名： | 国亚慧 于分弟 甘桂云 韦冠睦 王先裕 杜永臣 桝田正治 |
| 作者单位： | （;1. 广西大学农学院，广西南宁 530004；;2.广西来宾合山市岭南镇人民政府，广西来宾 546500；;3. 广西柳州市农业科学研究所，广西柳州 545003；;4. 中国农业科学院蔬菜花卉研究所，北京;100081；5 日本冈山大学，日本700 － 8530；;6.国家大宗蔬菜产业技术体系桂林综合试验站，广西;桂林 541004 ） |
| 基金项目： | 国家科技部国际合作项目(2006DFA33380);广西科技厅广西科学研究与技术开发计划项目(0718007B-38);南宁市青秀区科学技术局科学研究与技术开发计划项目(20080211B);桂林市局科学研究与技术开发计划(20100112-1);现代农业产业技术体系建设专项资金(CARS-25) |
| 摘 要： | 以温敏型核雄性不育系T-4 及对照First 为材料，采用考马斯亮蓝G-250 染色通过分光光度 法测定花药蛋白质含量，通过聚丙烯酰胺凝胶垂直板电泳分析POD 和EST 同工酶的酶谱差异。试验结果 表明，可育温度条件下的T-4 花药蛋白质含量显著高于不育温度处理，但与对照First 差异不显著。POD 及EST 同工酶酶谱显示，在不育温度条件下T-4 的POD 及EST 同工酶谱带均多于可育温度处理及对照 First；可育温度条件下T-4 的POD 及EST 同工酶谱带与对照First 差别不明显。 |
| 关 键 词： | 番茄 温敏型核雄性不育系 蛋白质 POD 同工酶 EST 同工酶 |
| 收稿时间： | 2012-11-04; |
Studies on Protein Content,POD and EST Isozyme of Thermo-sensitive Genic Male-sterile Tomato T-4 |
|
| GUO Ya-Hui,YU Fen-Di,GAN Gui-Yun,WEI Guan-Mu,WANG Xian-Yu,DU Yong-Chen,JIE Tian-Zheng-Zhi. Studies on Protein Content,POD and EST Isozyme of Thermo-sensitive Genic Male-sterile Tomato T-4[J]. China Vegetables, 2013, 1(6): 58-62 | |
| Authors: | GUO Ya-Hui YU Fen-Di GAN Gui-Yun WEI Guan-Mu WANG Xian-Yu DU Yong-Chen JIE Tian-Zheng-Zhi |
| Affiliation: | GUO Ya-hui 1,YU Fen-di 2,Gan Gui-yun 1,WEI Guan-mu 3,WANG Xian-yu 1,6*,DU Yongchen 4,Masaharu MASUDA 5(1 Agronomy College,Guangxi University,Nanning 530004,Guangxi,China;2 Government of Lingnan Town,Heshan City,Laibin 546500,Guangxi,China;3 Liuzhou City Institute of Agricultural Sciences,Liuzhou 545003,Guangxi,China;4 Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;5 Okayama University,700-8530,Japan;6 Guilin Comprehensive Experimental Station of National Staple Vegetable Industrial Technology Systems,Guilin 541004,Guangxi,China) |
| Abstract: | The experimental materials were thermo-sensitive genic male-sterile tomato（Lycopersicum esculentum Mill. ）T-4 and the control First．The protein content was determined by spectrophotometry， adopting Coomassiebrilliant blue G250 as dye．Meanwhile，the activity of POD and EST isozyme was analyzed with the polyacrylamide gel electrophoresis of perpendicular plate．Results showed that the protein content of T-4 anther under fertile condition was significantly higher than that under sterile condition，but was no significant difference with the control First．The band of POD and EST isozyme in anther of T-4 under sterile condition was more than that in anther of T-4 under fertile condition，and the control First．There was no significant difference in band of POD and EST isozyme between T-4 and the control First，when T-4 was under fertile situation. |
| Keywords: | Thermo-sensitive genic male-sterile Protein POD isozyme EST isozyme |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《中国蔬菜》浏览原始摘要信息 | |
| 点击此处可从《中国蔬菜》下载全文 | |