水稻SDG711蛋白C末端原核表达及多克隆抗体制备

目的]对水稻SDG711蛋白C末端进行原核表达,并制备其多克隆抗体。方法]选取水稻SDG711蛋白抗原决定簇较密集的C末端进行原核表达,通过构建原核表达载体pET28a-711C,转化E.coli BL21(DE3)感受态细胞,IPTG诱导表达融合蛋白后进行纯化,再以纯化的融合蛋白为抗原免疫新西兰白兔,制备多克隆抗体,并对其进行Western-blot分析。结果]试验制备的多克隆抗体能有效地检测抗原的表达。结论]该研究为进一步深入研究SDG711蛋白的功能奠定了基础。

Prokaryotic Expression and Polyclonal Antibody Preparation of SDG711 C-terminal from Rice

Objective] This study aimed to conduct prokaryotic expression of rice SDG711 C-terminal and prepare its polyclonal antibody. Method ] C-terminal of rice SDG711 containing relatively intensive antigen determinants was selected for prokaryotic expression, prokaryotic expression vector pET28a-711C was constructed and the recombinant plasmid was transformed into Escherichia coli BL21 （DE3） competent cells. The recombinant fusion protein was induced by IPTG and purified to immunize a New Zealand white rabbit as the antigen, and poly- clonal antibody was obtained for Westem-blot analysis. Result] The polyclonal antibody prepared could efficiently detect the antigen expres- sion. Conclusion] This study laid the foundation for further investigating the functions of SDG711 protein.