首页 | 本学科首页   官方微博 | 高级检索  
     检索      

酶抑制法检测蔬菜农药残留的效果评价
引用本文:叶雪珠,王强,赵首萍,章虎,王祥云,赵燕申.酶抑制法检测蔬菜农药残留的效果评价[J].中国蔬菜,2013,1(4):76-81.
作者姓名:叶雪珠  王强  赵首萍  章虎  王祥云  赵燕申
作者单位:浙江省农业科学院农产品质量标准研究所,农业部农药残留检测重点实验室,浙江杭州310021
基金项目:浙江省三农五方科技协作项目(2009R19A60D03);浙江省农业标准化重点研究项目(2011R19A60C01)
摘    要:采用色谱定量检测验证方法,对目前常用的酶抑制法速测蔬菜中农药残留的效果进行 了评
价。结果表明:酶抑制法检测不同农药灵敏度差异较大,其中以克百威的灵敏度最好,最低可达0.003
mg·kg-1,其次为灭多威和甲萘威。但对于目前蔬菜中常检出的毒死蜱和三唑磷等灵敏度不高。速测因最
低检测限高和基质干扰的影响,部分结果存在假阳性和假阴性风险,蔬菜产品假阳性率为7.1%~26.2%,
而以毒死蜱为试验农药的蔬菜样品检测假阴性率较高。因此,酶抑制法速测在蔬菜高毒快速控制上发挥了
一定作用,但作为执法检测需进一步研究前处理提取方法和提高 酶试剂质量。

关 键 词:酶抑制法  检测  农药残留  效果  
收稿时间:2012-08-29;

Effect Evaluation on Rapid Enzyme Inhibiting Method to Detect Pesticide Residues in Vegetable
YE Xue-zhu,WANG Qiang,ZHAO Shou-ping,ZHANG Hu,WANG Xiang-yun,ZHAO Yan-shen.Effect Evaluation on Rapid Enzyme Inhibiting Method to Detect Pesticide Residues in Vegetable[J].China Vegetables,2013,1(4):76-81.
Authors:YE Xue-zhu  WANG Qiang  ZHAO Shou-ping  ZHANG Hu  WANG Xiang-yun  ZHAO Yan-shen
Institution:Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agriculture Sciences, MOA Key Lab for; Pesticide Residue Detection, Hangzhou 310021, Zhejiang, China
Abstract:This paper evaluated the effect of daily used enzyme inhibition method to rapid detect
pesticide residues in vegetables,by chromatography quantitative detection.The results showed that
there were bigger differences in sensitivities of different pesticides using this method,among them sensitivity
of carbofuran was the best,and the lowest can reach 0.003 mg·kg-1,followed by methomyl and
carbaryl.But the sensitivities of chlorpyrifos and triazophos,which were often detected in present vegetables,
were not high.The rapid detection had false-positive and false-negative problems.The falsepositive
rates of vegetable products were 7.1%-26.2%,while the false-negative rates to test the pesticide
chlorpyrifos for vegetables sample were generally high.Therefore,the method of enzyme inhibition has
played certain role in rapid controlling high toxic vegetables,but as law enforcement detection,further
research is essential to improve the enzyme reagent quality and to study the pretreatment method.
Keywords:Detect  Pesticide residue  Effect
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《中国蔬菜》浏览原始摘要信息
点击此处可从《中国蔬菜》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号