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甘氨酸浓度对生姜组培苗氮代谢的影响
引用本文:廖林正,朱菲菲,刘奕清,唐建民,陈泽雄.甘氨酸浓度对生姜组培苗氮代谢的影响[J].中国蔬菜,2013,1(6):54-57.
作者姓名:廖林正  朱菲菲  刘奕清  唐建民  陈泽雄
作者单位:(;1.重庆文理学院花卉研究所,重庆市特色植物种苗工程技术研究中心,重庆402160;;2.西南大学; 园艺园林学院,重庆400716)
基金项目:重庆市科技创新能力建设项目(CSTC,2010CB1012);特色植物种苗工程重庆高校创新团队建设项目(201025);重庆高校优秀成果转化项目(Kjzh10214);重庆市教委重点科技项目(KJ12016);永川区科委重点攻关项目(YCSTC,2011AC1001)
摘    要:为探讨有机氮甘氨酸用量对生姜组培继代苗生理特性的影响,在无菌条件下以生姜组培苗为
试材,设置5 个不同甘氨酸浓度(1.0、1.5、2.0、2.5、3.0 mg·L-1),研究了生姜组培苗氮代谢相关产物
和氮代谢关键酶的活性。结果表明:生姜组培苗叶绿素含量、可溶性糖含量随着甘氨酸浓度的增加而上升,
可溶性蛋白含量变化趋势则相反;谷氨酰胺合成酶和谷草转氨酶活性亦随着甘氨酸浓度的增加而升高。

关 键 词:甘氨酸  生姜  组培苗  生理特性  
收稿时间:2012-10-23;

Effects of Glycine Concentrations on Nitrogen Metabolism in Plantlet in vitro of Ginger
LIAO Lin-zheng,ZHU Fei-fei,LIU Yi-qing,TANG Jian-min,CHEN Ze-xiong.Effects of Glycine Concentrations on Nitrogen Metabolism in Plantlet in vitro of Ginger[J].China Vegetables,2013,1(6):54-57.
Authors:LIAO Lin-zheng  ZHU Fei-fei  LIU Yi-qing  TANG Jian-min  CHEN Ze-xiong
Institution:(;1.Flower Research Institute, Chongqing University of Arts and Sciences, Chongqing Engineering Research Center; for Special Plant Seedlings, Chongqing 402160, China; ;2.Faculty of Horticulture and Landscape, Southwest University,; Chongqing 400716, China)
Abstract:In order to determine the effects of organic nitrogen glycine concentration on
physiological characteristics of ginger( Zingiber officinale Rosc.) plantlet in vitro,this paper
takes plantlet in vitro under aseptic cultivation as experimental material.Five kinds of glycine
concentration level( 1.0,1.5,2.0,2.5,3.0 mg·L-1) were set up to study the relevant product of
nitrogen metabolism of ginger plantlet in vitro and the activity of nitrogen metabolism key enzyme.
The results showed that the content of chlorophyll and soluble sugars raised with the increase of
glycine concentration,while that of soluble protein was contraire.The activity of GS and GOT were
strengthened with the increase of glycine concentration.
Keywords:Ginger  in vitro Plantlet in vitro  Physiological characteristics
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