| 抑制素质粒的提取与纯化研究 |
| |
| 引用本文： | 杨维,石国庆,夏洁,张红琳.抑制素质粒的提取与纯化研究[J].安徽农业科学,2009,37(23):10895-10896. |
| |
| 作者姓名： | 杨维 石国庆 夏洁 张红琳 |
| |
| 作者单位： | 南京晓庄学院行知学院,江苏南京,211171;新疆农垦科学院畜牧兽医所,新疆石河子,832000;南京晓庄学院生命科学系,江苏南京,211171 |
| |
| 基金项目： | 江苏省教育厅自然科学研究基金 |
| |
| 摘 要： | 目的]为抑制素质粒的大量提取与纯化寻找一种成本低的方法。方法]采用碱裂解法与DEAE-纤维柱层析法提取与纯化抑制素质粒DNA，用分光光度法测定其浓度和纯度。结果]抑制素质粒DNA的等电点为2．0～2．5，低于大部分蛋白质。pH值为8．0时，抑制素质粒DNA带负电荷，且电荷数量与RNA和蛋白质不同。用紫外分光光度计测定的柱层析后洗脱峰1、2、3在260和280nm波长处的吸光度值分别为3．01和2．9、0．95和0．51、0．84和0．76，其OD260nm/OD280nm分别为1．04、1．86和1．10。3mol／L NaCl的洗脱峰1大部分是蛋白质，0．6mol／L NaCl的洗脱峰2是浓度为93．0ng／ml的抑制素质粒DNA，3mol／L NaCl的洗脱峰3是杂质。结论]利用碱裂解法能从大肠杆菌细胞中分离出抑制素质粒DNA，DEAE-纤维柱层析法纯化的抑制素质粒DNA达到了要求的纯度。
|
| 关 键 词： | 质粒 提取 纯化 碱裂解法 DEAE-纤维柱层析 |
Study on Extraction and Purification of Inhibin Plasmid |
| |
| YANG Wei et al.Study on Extraction and Purification of Inhibin Plasmid[J].Journal of Anhui Agricultural Sciences,2009,37(23):10895-10896. |
| |
| Authors: | YANG Wei |
| |
| Institution: | YANG Wei et al(Xingzhi college,Nanjing Xiaozhuang University,Nanjing,Jiangsu 211171) |
| |
| Abstract: | Objective] The purpose was to find a low-cost method for mass extraction and purification of inhibin plasmid.Method] The inhibin plasmid DNA was extracted and purified by alkaline lysis method and DEAE-fiber column chromatography and its concn.and purity were determined by spectrophotometry.Result] The isoelectric point of inhibin plasmid DNA was 2.0-2.5 and it was lower than that of most proteins.When pH value was 8.0,the inhibin plasmid DNA was negatively charged and its charge quantity was different f... |
| |
| Keywords: | Plasmid Extraction Purification Alkaline lysis method DEAE-fiber column chromatography |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
