| 离体萌发法测定垂丝海棠花粉生活力的研究 |
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| 引用本文： | 刘会超,贾文庆.离体萌发法测定垂丝海棠花粉生活力的研究[J].山西农业科学,2007,35(5):39-41. |
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| 作者姓名： | 刘会超 贾文庆 |
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| 作者单位： | 河南科技学院园林学院,河南,新乡,453003 |
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| 基金项目： | 河南省高校杰出科研创新人才工程项目;河南科技学院校科研和教改项目 |
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| 摘 要： | 应用离体萌发法对垂丝海棠的花粉生活力进行测定,同时研究了不同浓度的钙对花粉萌发和花粉管生长的影响。研究结果表明,垂丝海棠花粉萌发和花粉管生长的最佳培养基是:0.8 g琼脂+15 mg/L蔗糖+2.0 mg/L硼酸;低浓度的钙对花粉萌发和花粉管的生长有促进作用,高浓度的钙对花粉萌发和花粉管的生长有抑制作用;培养基中的Ca(NO3)2浓度为1.6×10-5mg/L时最适合垂丝海棠花粉萌发和花粉管的生长。
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| 关 键 词： | 垂丝海棠 花粉 生活力 测定方法 |
| 文章编号： | 1002-2481（2007）05-0039-03 |
| 修稿时间： | 2007-06-21 |
Determination on Pollen Viability of Malus Halliana |
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| LIU Hui-chao,JIA Wen-qing.Determination on Pollen Viability of Malus Halliana[J].Journal of Shanxi Agricultural Sciences,2007,35(5):39-41. |
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| Authors: | LIU Hui-chao JIA Wen-qing |
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| Institution: | Department of Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003 , China |
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| Abstract: | The viability of pollen of Malus halliana was studied with the methods in which the germination was in vitro,and the influence of the different concentration of calcium on pollen germination and pollen tube growth was studied.The results showed that,the best culture medium that was advantageous in pollen germination and pollen tubes growth was:0.8g agar +15 mg/L sucrose +2.0 m/L boric acid;low concentration of calcium had a good effect on pollen germination and pollen tube growth,and high concentration of calcium was inhibited to them.The most suitable concentration of calcium to promote pollen germination and pollen tubes growth was 1.6×10-5 mg/L in the cultural medium. |
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| Keywords: | Malus halliana Pollen Viability Assay method |
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