Comparison of the genetic structure of populations of wild emmer wheat, <Emphasis Type="Italic">Triticum turgidum</Emphasis> ssp. <Emphasis Type="Italic">dicoccoides</Emphasis>, from Israel and Turkey revealed by AFLP analysis |
| |
Authors: | O Ozbek E Millet Y Anikster O Arslan M Feldman |
| |
Institution: | (1) Department of Plant Sciences, The Weizmann Institute of Science, Rehovot, 76100, Israel;(2) Institute for Cereal Crops Improvement, Tel Aviv University, Tel Aviv, Israel;(3) Institute of Science and Technology, Gazi University, Ankara, Turkey |
| |
Abstract: | The aim of the present study was to assess the genetic variation in several Israeli and Turkish populations of wild emmer
wheat, Triticum turgidum ssp. dicoccoides, the progenitor of most domesticated wheat. Single spikes were collected in 2002 from 60 plants that grew in six different
habitats in Ammiad, northeastern Israel (8–12 plants from each habitat), and in 1998 from 56 plants that grew in seven different
habitats in Diyarbakir, southeastern Turkey (8 plants from each habitat). Seeds were planted in a nursery and DNA was extracted
from every plant and analyzed by the fluorescent-based amplified fragment length polymorphism (AFLP) method. Seven primer
combinations produced 788 discernible loci of which 48.6% were polymorphic in Israel and 40.5% in Turkey. The genetic diversity
estimates P (frequency of polymorphic loci) and He (gene diversity) were higher in Ammiad than in Diyarbakir (means of P = 0.34 and He = 0.13 in Ammiad vs. P = 0.20 and He = 0.08 in Diyarbakir). Ammiad populations contained more unique alleles than Diyarbakir populations. The relative genetic
diversity estimates (θ) values were 0.188 in Ammiad and 0.407 in Diyarbakir, suggesting better differentiation of the populations in Turkey. Genetic
distance was larger between Israeli and Turkish populations than between populations of each country. The data indicate that
the Israeli and Turkish populations are considerably diverged and that the Israeli populations are more polymorphic than the
Turkish ones, having a larger within-populations genetic variation than among-populations one. The significance of the results
in relation to the differentiation pattern of wild emmer in the Near East is discussed. |
| |
Keywords: | AFLP Genetic variation Israeli and Turkish populations Population structure Triticum turgidum ssp dicoccoides Wild emmer wheat |
本文献已被 SpringerLink 等数据库收录! |
|