Development of optimal conditions for lymphokine production by chicken lymphocytes |
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| Authors: | H Weiler V von Bülow |
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| Institution: | 1. Institut of Veterinary Pathology, Free University Berlin, Strasse 518 Nr. 15, D-1000 Berlin 37, W. Germany;2. Institut of Poultry Diseases, Free University Berlin, Koserstrasse 21, D-1000 Berlin 33, W. Germany;1. Department of Agriculture, Graduate School of Science and Technology, Shinshu University, Japan;2. Department of Agricultural and Life Science, Faculty of Agriculture, Shinshu University, Japan;3. Department of Interdisciplinary Genome Sciences and Cell Metabolism, Institute for Biomedical Sciences, Shinshu University, Japan;1. Department of Animal Science, College of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China;2. Department of Veterinary Medicine, College of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China;1. Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India;2. Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore 721102, West Bengal, India;3. Regional Plant Resource Centre, Bhubaneswar 751015, Odisha, India;1. Moredun Research Institute, Pentlands Science Park, Penicuik, Midlothian, EH26 0PZ, United Kingdom;2. Biomathematics and Statistics Scotland, James Clerk Maxwell Building, The King''s Buildings, Peter Guthrie Tait Road, Edinburgh, EH9 3FD, United Kingdom;1. Department of Environmental and Occupational Health, College of Medicine, National Cheng Kung University, Tainan 70428, Taiwan;2. Institute for Structural Biology, Drug Discovery and Development and Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, VA, United States |
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| Abstract: | Chicken thymus, spleen, and bursa lymphocytes were isolated by different methods and incubated under differing conditions in order to obtain and characterize avian lymphokines. The biological activity of lymphokine-containing cell culture supernatants was measured by their antiviral activity (interferon(IFN)-units) and by their capacity to induce cytostatic effects in bone-marrow-derived macrophages (50% cytostasis-inducing dose, CID). Lymphokine production by thymus lymphocytes required concanavalin A (ConA)-stimulation, while spleen cells, when cultured at high density, released CID and IFN activities into the culture medium even without mitogen-stimulation. By way of comparison, the highest lymphokine content was found in the supernatant of lymphocyte cultures, which were incubated for 72 hours at 41 degrees C after stimulation with an optimal ConA dose. For stimulation of thymus lymphocytes 30 micrograms ConA/ml were found to be optimal, independent of serum content and cell density in the cultures. In contrast, the optimal ConA dose for spleen lymphocytes not only depended on the serum content but also on the cell density in the cultures and varied within a range of 2.5 micrograms and 45 micrograms ConA/ml. |
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